Searching journal content for articles similar to Hilson et al. 14 (10b): 2176.

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  1. Perspective: Challenges in identifying mRNA transcript starts and ends from long-read sequencing data
    • Ezequiel Calvo-Roitberg,
    • Rachel F. Daniels,
    • and Athma A. Pai
    Genome Res. November 2024 34: 1719-1734; doi:10.1101/gr.279559.124
    ...application. Oligo(dT) primers enrich for mature RNAs transcribed by RNAPII by annealing to the polyadenylated tail present at the 3′ end of most eukaryotic mRNA molecules. Random hexamers anneal to various sites along the RNA molecules, allowing for reverse transcription of the entire RNA population starting...
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  2. Methods: A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices
    • Jian-Bing Fan,
    • Joanne M. Yeakley,
    • Marina Bibikova,
    • Eugene Chudin,
    • Eliza Wickham,
    • Jing Chen,
    • Dennis Doucet,
    • Philippe Rigault,
    • Baohong Zhang,
    • Richard Shen,
    • Celeste McBride,
    • Hai-Ri Li,
    • Xiang-Dong Fu,
    • Arnold Oliphant,
    • David L. Barker,
    • and Mark S. Chee
    Genome Res. May 2004 14: 878-885; doi:10.1101/gr.2167504
    ...gene, for 96 samples in parallel. The c D NA-mediated a nnealing, s election, extension and l igation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained...
  3. PERSPECTIVE: The Arabidopsis genome: A foundation for plant research
    • Michael Bevan and
    • Sean Walsh
    Genome Res. December 2005 15: 1632-1642; doi:10.1101/gr.3723405
    ...the resolution of array analysis to the cellular level. Recently, Zanetti et al. ( 2005 ) immunopurified polysomes using an epitope-tagged ribosomal protein and showed that transcript profiles obtained by RNA isolated from these polysomes yielded highly reproducible data. Thus it may be possible to express...
  4. Review: A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest
    • Andrew D. Bates,
    • Dawid Grzela,
    • Maciej Studzian,
    • Louise Brennan,
    • Moli Williams,
    • Conor Fawcett,
    • Beth Hammond,
    • Manreen Grewal,
    • Marcin Ratajewski,
    • Lukasz Pulaski,
    • and Urszula L. McClurg
    Genome Res. March 2026 36: 433-459; Published in Advance February 17, 2026, doi:10.1101/gr.281154.125
    ...on potentially misleading predictions.Prediction of potential regulatory sitesThis review focuses on gene-specific transcriptional regulation via direct TF binding to regulatory DNA sites. The initial task is thus to locate candidate DNA regions potentially involved in TF binding, and this starts...
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  5. PERSPECTIVE: ORFeome Cloning and Systems Biology: Standardized Mass Production of the Parts From the Parts-List
    • Michael A. Brasch,
    • James L. Hartley,
    • and Marc Vidal
    Genome Res. October 15, 2004 14: 2001-2009; doi:10.1101/gr.2769804
    .... ↵ Hilson, P., Allemeersch, J., Altmann, T., Aubourg, S., Avon, A., Beynon, J., Bhalerao, R.P., Bitton, F., Caboche, C., Cannoot, B., et al. 2004 . Versatile gene-specific sequence tags for Arabidopsis functional genomics: Transcript profiling and reverse genetics applications. Genome Res. (this issue...
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