Searching journal content for articles similar to Hillier et al. 19 (4): 657.

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  1. ...Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus Hinxton, Cambridge CB10 1SA, United Kingdom Corresponding author: ana.conesa@csic.esAbstractLong-read sequencing (LRS) technologies have revolutionized transcriptomic research by enabling the comprehensive sequencing of full...
  2. ...These authors contributed equally to this work. Corresponding author: zyzhao@hkbu.edu.hkAbstractMassively parallel sequencing of the polyadenylated RNAs has played a key role in delineating transcriptome complexity, including alternative use of an exon, promoter, 5′ or 3′ splice site or polyadenylation site...
  3. ...nanopore-based direct RNA sequencing to characterize the developmental polyadenylated transcriptome of C. elegans. Taking advantage of long reads spanning the full length of mRNA transcripts, we provide support for 23,865 splice isoforms across 14,611 genes, without the need for computational...
  4. ...The time-resolved transcriptome of C. elegans Max E. Boeck 1 , 2 , Chau Huynh 1 , Lou Gevirtzman 1 , Owen A. Thompson 1 , Guilin Wang 3 , Dionna M. Kasper 3 , Valerie Reinke 3 , LaDeana W. Hillier 1...
  5. ...Sciences, Hanyang University, Seoul 133791, Republic of Korea The advent of high-throughput RNA sequencing (RNA-seq) has led to the discovery of unprecedentedly immense transcriptomes encoded by eukaryotic s. However, the transcriptome maps are still incomplete partly because they were mostly reconstructed...
  6. ...Chromatin using sequencing. We optimized the ATAC-seq protocol for C. elegans by including a step of native nuclei isolation by mechanical homogenization before the transposition step (see Methods; Supplemental Extended Protocol). The low input requirements of ATAC-seq (several orders of magnitude less than...
  7. ...of translational efficiency. In Caenorhabditis elegans, the majority of genes are spliced leader (SL) trans-spliced (Blumenthal 2005; Allen et al. 2011). In this process, an SL RNA trims the 5′ UTR of pre-mRNA and then attaches a short (∼22 nucleotide [nt]) sequence to the 5′ terminus (Krause and Hirsh 1987...
  8. ...-scale DNA sequencing technologies have made the study of the polyadenylated transcriptome easier. The digital gene expression (DGE) protocol sequenced by massively parallel signature sequencing (MPSS) or Illumina’s GAII-based sequencing-by-synthesis (SBS) (Meyers et al. 2004c; Simon et al. 2009), called...
  9. .... elegans. Nature 541: 102–106. ↵Hillier LW, Reinke V, Green P, Hirst M, Marra MA, Waterston RH. 2009. Massively parallel sequencing of the polyadenylated transcriptome of C. elegans. Genome Res 19: 657–666. ↵Jaillon O, Bouhouche K, Gout JF, Aury JM, Noel B, Saudemont B, Nowacki M, Serrano V, Porcel BM...
  10. ...Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells Ryan D. Morin 1 , Michael D. O’Connor 2 , Malachi Griffith 1 , Florian Kuchenbauer 2 , Allen Delaney 1 , Anna-Liisa Prabhu 1...
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