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  1. ...and names of each chromosome rearrangement identified are represented by blue (TDDO) and orange (deletion) arrows along chromosomes. Characteristics of each TDDO can be found in Supplemental Table S2. (B) CNV of genes present or not in TDDO3 and of rRNA genes were determined by quantitative PCR...
  2. ...16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res 21: 494–504. ↵ Han YW, Shi W, Huang GT, Kinder Haake S, Park NH, Kuramitsu H, Genco RJ Han YW, Shi W, Huang GT, Kinder Haake S, Park NH, Kuramitsu H, Genco RJ. 2000. Interactions between periodontal...
  3. ..., contigs with sizes of 605–46,278 bp (URFT1 strain), rRNA operons, and CDSs (LVS strain). On the other hand, a total of 377 short remaining contigs (81–425 bp) were rejected by the bioinformatic tools used for the four Francisella s. However, using BLASTN analysis ( E -value = 10 −4 ), a first subset...
  4. ...-range PCR step to generate an amplicon target for long-read sequencing. The 16S rRNA gene is a conserved genetic marker that is present in nearly all bacteria and archaea, and its sequencing allows for the identification of microorganisms based on the sequence similarity of their 16S rRNA gene (Tringe...
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  5. ...an exceptional bargain compared to Sanger sequencing of full-length amplicons. However, to define new bacterial phyla, or in cases in which the sequences obtained are highly divergent from related sequences in the reference databases, obtaining the full-length sequence (e.g., by repeating the PCR using a very...
  6. ...the highly conserved regions used for primer design may amplify less efficiently or not amplify at all. For example, the 16S rRNA gene sequence of the Nanoarchaeota is so divergent that PCR with the “universal” primers failed to detect this species even from cultured organisms ( Huber et al. 2002 ). Second...
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