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RESEARCH: An STS-Based Radiation Hybrid Map of the Human Genome
- Elizabeth A. Stewart,
- Kathleen B. McKusick,
- Amita Aggarwal,
- Ewa Bajorek,
- Shannon Brady,
- Angela Chu,
- Nicole Fang,
- David Hadley,
- Mark Harris,
- Sami Hussain,
- Richard Lee,
- Annu Maratukulam,
- Kyle O’Connor,
- Shanti Perkins,
- Mark Piercy,
- Fawn Qin,
- Tim Reif,
- Carla Sanders,
- Xiaohong She,
- Wei-Lin Sun,
- Poroshat Tabar,
- Susan Voyticky,
- Sid Cowles,
- Jian-Bing Fan,
- Chris Mader,
- John Quackenbush,
- Richard M. Myers,
- and David R. Cox
Genome Res. May 1, 1997 7: 422-433; doi:10.1101/gr.7.5.422
...is an important goal of the U.S. Human Genome Project. The construction of such a map has been greatly facilitated in recent years by the development of sequence-tagged sites (STSs) as genomic landmarks ( Olson et al. 1989 ). Each STS is defined by a short segment of human DNA sequence and is assayed by means...
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ARTICLE: X chromosome map at 75-kb STS resolution, revealing extremes of recombination and GC content.
- R Nagaraja,
- S MacMillan,
- J Kere,
- C Jones,
- S Griffin,
- M Schmatz,
- J Terrell,
- M Shomaker,
- C Jermak,
- C Hott,
- M Masisi,
- S Mumm,
- A Srivastava,
- G Pilia,
- T Featherstone,
- R Mazzarella,
- S Kesterson,
- B McCauley,
- B Railey,
- F Burough,
- V Nowotny,
- M D'Urso,
- D States,
- B Brownstein,
- and D Schlessinger
Genome Res. March 1997 7: 210-222; doi:10.1101/gr.7.3.210
...: 546 ; 563 – 567 . Green, E.D. , Green. P. Green, E.D. and Green. P. 1991 . Sequence-tagged site (STS) content mapping of human chromosomes: Theoretical considerations and early experiences. PCR Methods Applic. 1 : 77 – 90 . Green, E.D. , Olson. M.V. Green, E.D. and Olson. M.V. 1990 . Chromosomal...
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RESEARCH: A Physical Map of Human Chromosome 7: An Integrated YAC Contig Map with Average STS Spacing of 79 kb
- Gerard G. Bouffard,
- Jacquelyn R. Idol,
- Valerie V. Braden,
- Leslie M. Iyer,
- Aimee F. Cunningham,
- Lauren A. Weintraub,
- Jeffrey W. Touchman,
- Rose M. Mohr-Tidwell,
- Dale C. Peluso,
- Robert S. Fulton,
- Melinda S. Ueltzen,
- Jean Weissenbach,
- Charles L. Magness,
- and Eric D. Green
Genome Res. July 1, 1997 7: 673-692; doi:10.1101/gr.7.7.673
...to an estimated 5% of the human genome. Using a yeast artificial chromosome (YAC)-based sequence-tagged site (STS)-content mapping strategy, 2150 chromosome 7-specific STSs have been established and mapped to a collection of YACs highly enriched for chromosome 7 DNA. The STSs correspond to sequences generated...
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ARTICLES: Eliminating mitochondrial DNA competition for nuclear DNA primers.
- S Zullo,
- J L Kennedy,
- J Gelernter,
- M H Polymeropoulos,
- G Tallini,
- A J Pakstis,
- M B Shapiro,
- C R Merril,
- and K K Kidd
Genome Res. August 1993 3: 39-45
...Laboratory of Biochemical Genetics, National Institute of Mental Health Neuroscience Center at St. Elizabeth, Washington, D.C. 20032. Abstract Mitochondrial DNA (mtDNA) sequences were synthesized with nuclear DNA (nucDNA) sequence-tagged site (STS) primers by mismatch priming in three independent...
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LETTER: Contig Maps and Genomic Sequencing Identify Candidate Genes in the Usher 1C Locus
- Michael J. Higgins,
- Colleen D. Day,
- Nancy J. Smilinich,
- L. Ni,
- Paul R. Cooper,
- Norma J. Nowak,
- Chris Davies,
- Pieter J. de Jong,
- Fielding Hejtmancik,
- Glen A. Evans,
- Richard J.H. Smith,
- and Thomas B. Shows
Genome Res. January 1, 1998 8: 57-68; doi:10.1101/gr.8.1.57
.... , McGillivray B.C. , Hayden M. , Jaschek G. , Bruns G.A.P. ( 1989 ) A deletion map of the WAGR region on chromosome 11. Am. J. Hum. Genet. 44 : 486 – 495 . ↵ Green E.D. , Green P. ( 1991 ) Sequence-tagged site (STS) content mapping of human chromosomes: Theoretical considerations and early experiences...
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LETTER: An Integrated Physical Map for the Short Arm of Human Chromosome 5
- Ellen T. Peterson,
- Robert Sutherland,
- Donna L. Robinson,
- Leslie Chasteen,
- Meryl Gersh,
- Joan Overhauser,
- Larry L. Deaven,
- Robert K. Moyzis,
- and Deborah L. Grady
Genome Res. December 1, 1999 9: 1250-1267; doi:10.1101/gr.9.12.1250
...and regional localization of 303 new chromosome 5 sequence-tagged sites. Genomics 32 : 91 – 96 . ↵ Green E.D. , Green P. ( 1991 ) Sequence-tagged site (STS) content mapping of human chromosomes: Theoretical considerations and early experiences. PCR Methods Applic. 1 : 77 – 90 . ↵ Gyapay G. , Schmitt K...
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LETTER: From First Base: The Sequence of the Tip of the X Chromosome of Drosophila melanogaster, a Comparison of Two Sequencing Strategies
- Panayiotis V. Benos,
- Melanie K. Gatt,
- Lee Murphy,
- David Harris,
- Bart Barrell,
- Concepcion Ferraz,
- Sophie Vidal,
- Christine Brun,
- Jacques Demaille,
- Edouard Cadieu,
- Stephane Dreano,
- Stéphanie Gloux,
- Valerie Lelaure,
- Stephanie Mottier,
- Francis Galibert,
- Dana Borkova,
- Belen Miñana,
- Fotis C. Kafatos,
- Slava Bolshakov,
- Inga Sidén-Kiamos,
- George Papagiannakis,
- Lefteris Spanos,
- Christos Louis,
- Encarnación Madueño,
- Beatriz de Pablos,
- Juan Modolell,
- Annette Peter,
- Petra Schöttler,
- Meike Werner,
- Fotini Mourkioti,
- Nicole Beinert,
- Gordon Dowe,
- Ulrich Schäfer,
- Herbert Jäckle,
- Alain Bucheton,
- Debbie Callister,
- Lorna Campbell,
- Nadine S. Henderson,
- Paul J. McMillan,
- Cathy Salles,
- Evelyn Tait,
- Phillipe Valenti,
- Robert D.C. Saunders,
- Alain Billaud,
- Lior Pachter,
- David M. Glover,
- and Michael Ashburner
Genome Res. May 1, 2001 11: 710-730; doi:10.1101/gr.173801
...bands 3A2 and 3C2, believed in the 1970s to show a correlation between the number of bands on the polytene chromosomes and the 20 genes identified by conventional genetics, is predicted to contain 45 genes from its DNA sequence. We have determined the insertion sites of P-elements from 111 mutant lines...
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LETTER: A collection of 1814 human chromosome 7-specific STSs.
- G G Bouffard,
- L M Iyer,
- J R Idol,
- V V Braden,
- A F Cunningham,
- L A Weintraub,
- R M Mohr-Tidwell,
- D C Peluso,
- R S Fulton,
- M P Leckie,
- and E D Green
Genome Res. January 1997 7: 59-64; doi:10.1101/gr.7.1.59
.... Green, E.D. and Green. P. 1991 . Sequence-tagged site (STS) content mapping of human chromosomes: Theoretical considerations and early experiences. PCR Methods Applic. 1 : 77 – 90 . Green, E.D. , Olson. M.V. Green, E.D. and Olson. M.V. 1990a . Chromosomal region of the cystic fibrosis gene in yeast...
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ARTICLE: Mapping the RP10 locus for autosomal dominant retinitis pigmentosa on 7q: refined genetic positioning and localization within a well-defined YAC contig.
- R E McGuire,
- S A Jordan,
- V V Braden,
- G G Bouffard,
- P Humphries,
- E D Green,
- and S P Daiger
Genome Res. April 1996 6: 255-266; doi:10.1101/gr.6.4.255
...site (STS) content mapping of human chromosomes: Theoretical considerations and early experiences. PCR Methods Applic. 1 : 77 – 90 . Green, E.D. and Olson. M.V. 1990 . Systematic screening of yeast artifical chromosome libraries by use of the polymerase chain reaction. Proc. Natl. Acad. Sci. 87 : 213...
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Methods: ECgene: Genome-based EST clustering and gene modeling for alternative splicing
- Namshin Kim,
- Seokmin Shin,
- and Sanghyuk Lee
Genome Res. April 2005 15: 566-576; doi:10.1101/gr.3030405
...(Gene modeling by EST Clustering), which combines genome-based EST clustering and the transcript assembly procedure in a coherent and consistent fashion. Specifically, ECgene takes alternative splicing events into consideration. The position of splice sites (i.e., exon–intron boundaries) in the genome...

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