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Research: Distinct global shifts in genomic binding profiles of limb malformation-associated HOXD13 mutations
- Daniel M. Ibrahim,
- Peter Hansen,
- Christian Rödelsperger,
- Asita C. Stiege,
- Sandra C. Doelken,
- Denise Horn,
- Marten Jäger,
- Catrin Janetzki,
- Peter Krawitz,
- Gundula Leschik,
- Florian Wagner,
- Till Scheuer,
- Mareen Schmidt-von Kegler,
- Petra Seemann,
- Bernd Timmermann,
- Peter N. Robinson,
- Stefan Mundlos,
- and Jochen Hecht
Genome Res. December 2013 23: 2091-2102; Published in Advance August 30, 2013, doi:10.1101/gr.157610.113
...wild-type and mutant HOXD13 homeodomains was performed using fluorescent dye-labeled double-stranded oligonucleotides containing a binding sequence for HOXD13 (CCAATAAAA). Oligonucleotides were incubated with purified wild-type and mutant HOXD13 homeodomains. Unlabeled competitor oligonucleotides...
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Methods: Mapping translocation breakpoints by next-generation sequencing
- Wei Chen,
- Vera Kalscheuer,
- Andreas Tzschach,
- Corinna Menzel,
- Reinhard Ullmann,
- Marcel Holger Schulz,
- Fikret Erdogan,
- Na Li,
- Zofia Kijas,
- Ger Arkesteijn,
- Isidora Lopez Pajares,
- Margret Goetz-Sothmann,
- Uwe Heinrich,
- Imma Rost,
- Andreas Dufke,
- Ute Grasshoff,
- Birgitta Glaeser,
- Martin Vingron,
- and H. Hilger Ropers
Genome Res. July 2008 18: 1143-1149; Published in Advance March 7, 2008, doi:10.1101/gr.076166.108
...or inactivating specific genes, and the characterization of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome breakpoints using traditional methods, such as in situ hybridization with fluorescent dye-labeled...
REVIEW: The ligase chain reaction in a PCR world.
- F Barany
Genome Res. August 1991 1: 5-16; doi:10.1101/gr.1.1.5
.... Enzymatic repair of DNA. II. Characterization of phage-induced sealase. Proc. Natl. Acad. Sci. 58 : 1996 – 2003 . 52. Yudelevich, A., , Ginsberg, B. , Hurwitz. J. Yudelevich, A., Ginsberg, B. and Hurwitz. J. 1968 . Discontinuous synthesis of DNA during replication. Proc. Natl. Acad. Sci. 61 : 1129 – 1136...
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ARTICLE: Mutation detection by fluorescent chemical cleavage: application to hemophilia B.
- I I Haris,
- P M Green,
- D R Bentley,
- and F Giannelli
Genome Res. April 1994 3: 268-271
...Acids Res. 16 : 1215 . 8.. Giusti, W.G. , Adriano. T. Giusti, W.G. and Adriano. T. 1993 . Synthesis and characterization of 5′-fluorescent dye labeled oligonucleotides. PCR Methods Applic. 2 : 223 – 227 . 9.. Theisen, P., , McCollum, C. , Upadhya, K. , Jacobsen, K. , Vu, H.N. , Andrus. A. Theisen, P...
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METHODS: SNP Genotyping by Multiplexed Solid-Phase Amplification and Fluorescent Minisequencing
- Michael H. Shapero,
- Kerstin K. Leuther,
- Anhthu Nguyen,
- Melissa Scott,
- and Keith W. Jones
Genome Res. November 1, 2001 11: 1926-1934; Published in Advance October 15, 2001, doi:10.1101/gr.205001
...in one of the amplification primers, and digestion with the type IIS enzyme exposes the polymorphic site in such a way that it can be interrogated directly via fluorescent dye-labeled nucleotide incorporation. No additional synthesis of locus-specific oligonucleotides is required for the detection...
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ARTICLE: A one-step coupled amplification and oligonucleotide ligation procedure for multiplex genetic typing.
- F A Eggerding
Genome Res. June 1995 4: 337-345
..., W.G. and Adriano. T. 1993 . Synthesis and characterization of 5′-fluorescent-dye-labeled oligonucleotides. PCR Methods Applic. 2 : 223 – 227 . 23.. Barany, F. , Gelfand. D.H. Barany, F. and Gelfand. D.H. 1991 . Cloning, overexpression and nucleotide sequence of a thermostable DNA ligase...
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RESOURCE: Temperature-Modulated Array High-Performance Liquid Chromatography
- Andreas Premstaller,
- Wenzhong Xiao,
- Herbert Oberacher,
- Matthew O'Keefe,
- David Stern,
- Thomas Willis,
- Christian G. Huber,
- and Peter J. Oefner
Genome Res. November 1, 2001 11: 1944-1951; doi:10.1101/gr.200401
.... Both unlabeled and HPLC purified fluorescent dye-labeled oligonucleotides were obtained from either Applied Biosystems or Life Technologies. PCR Amplifications were performed in 50 μL reactions that contained 1 unit of AmpliTaq Gold (Applied Biosystems), 10 mM Tris·HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl2...

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