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  1. ...the specificity of three major approaches to high-throughput array-based DNA methylation analysis: (1) MeDIP, or methylated DNA immunoprecipitation, an example of antibody-mediated methyl-specific fractionation; (2) HELP, or HpaII tiny fragment enrichment by ligation-mediated PCR, an example of differential...
  2. ...High-throughput DNA methylation profiling using universal bead arrays Marina Bibikova 1 , Zhenwu Lin 2 , Lixin Zhou 1 , Eugene Chudin 1 , Eliza Wickham Garcia 1 , Bonnie Wu 1 , Dennis Doucet 1 , Neal J. Thomas 3...
  3. ...provides vital information on genomic locations of cancer-specific DMRs that may now facilitate high-throughput screening analyses for prostate cancer disease markers. Moreover, future -wide analyses of DNA methylation may improve with larger patient sample sets and with the incorporation ofmultiple...
  4. ....MethyLight: A high-throughput assay to measure DNA methylation. Nucleic Acids Res 28: e32. http:// nar.oxfordjournals.org/cgi/content/abstract/28/8/e32. Ehrich M, Nelson MR, Stanssens P, Zabeau M, Liloglou T, Xinarianos G, Cantor CR, Field JK, van den Boom D. 2005. Quantitative highthroughput analysis...
  5. ...such complex processes. In this review we discuss new approaches being used to identify sites of cytosine methylation throughout s that have been made possible by rapid advances in high-throughput DNA sequencing and discuss some of the challenges encountered and that lie ahead. Initial studies of single...
  6. .... In autoimmune diseases, environmentally driven epigenetic changes are thought to contribute to their etiology. Here we report the first high-throughput and candidate sequence analyses of DNA methylation to investigate discordance for autoimmune disease in twins. We used a cohort of MZ twins discordant for three...
  7. ...I representation provides a robust control with which to compare the HpaII representation. The HELP assay can be used on any genomic microarray and with any combination of restriction enzyme isoschizomers differing in sensitivity to cytosine methylation of the target sequence. By using a customized oligonucleotide...
  8. ....L. , Paul, C.L. ( 1992 ) A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands . Proc. Natl. Acad. Sci. 89 : 1827 – 1831 . ↵ Gitan, R.S. , Shi, H. , Chen, C.M. , Yan, P.S. , Huang, T.H. ( 2002 ) Methylation-specific oligonucleotide microarray...
  9. ...for multiplex detection of low abundance point mutations. J. Mol. Biol. 292 : 251 -262. ↵ Gitan, R.S., Shi, H., Chen, C.M., Yan, P.S., and Huang, T.H. 2002 . Methylation-specific oligonucleotide microarray: A new potential for high-throughput methylation analysis. Genome Res. 12 : 158 -164. ↵ Gonzalgo, M...
  10. ...approaches preceding the availability of structural data. PWM-based predictions depend on databases like TRANSFAC (Matys et al. 2006) and JASPAR (Khan et al. 2018). Modern high-throughput techniques that experimentally identify protein–DNA binding, such as ChIP-seq, SELEX, and protein-binding microarrays...
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