Searching journal content for articles similar to George et al. 11 (10): 1780.

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  1. ...with the availability of sensitive and rapid mutation detection platforms such as automated capillary sequencers and denaturing high-performance liquid chromatography (HPLC; Medintz et al. 2001 ; Liu et al. 2002 ), the routine sequencing of 16.5 kb of mitochondrial DNA is an onerous task. Microarrays are inherently...
  2. ...DNaseI treated sample that had not been enriched by ligation-mediated primer extension. This input was labeled with Cyanine 5 dCTP (Perkin Elmer). Microarray fabrication The Tal1 and α-globin tiling path microarrays were fabricated as detailed ( Dhami et al. 2005 , 2006 ). In brief, primer pairs were...
  3. ...Classification of a large microarray data set: Algorithm comparison and analysis of drug signatures Georges Natsoulis 1 , 4 , Laurent El Ghaoui 2 , Gert R.G. Lanckriet 2 , Alexander M. Tolley 1 , Fabrice Leroy 3 , Shane Dunlea 1...
  4. ...Representational Oligonucleotide Microarray Analysis: A High-Resolution Method to Detect Genome Copy Number Variation Robert Lucito 1 , 5 , John Healy 1 , Joan Alexander 1 , Andrew Reiner 1 , Diane Esposito 1 , Maoyen Chi 1...
  5. ....R. , Chandler D.P. , Brockman F. ( 2001 ) Fabrication of DNA microarrays using unmodified oligonucleotide probes. BioTechniques 30 : 368 – 372 . ↵ Chee M. , Yang R. , Hubbell E. , Berno A. , Huang X.C. , Stern D. , Winkler J. , Lockhart D.J. , Morris M.S. , Fodor S.P. ( 1996 ) Accessing genetic information...
  6. ...Assessing the performance of different high-density tiling microarray strategies for mapping transcribed regions of the human genome Olof Emanuelsson 1 , 6 , Ugrappa Nagalakshmi 2 , Deyou Zheng 1 , Joel S. Rozowsky 1 , Alexander E. Urban 2...
  7. ...) and followed by RNeasy Midi-RNA preparation kit (Qiagen) ( http://oncwebl.onc.jhmi.edu/microarray ). Microarray fabrication and hybridization The cDNA inserts from EST clones were amplified by PCR using universal M13 forward and M13 reverse primers, purified using Montage PCR96 purification kit (Millipore...
  8. ...• SSC (20 • SSC = 3 M NaC1, 0.3 M Na 3 citrate) in preparation for spotting onto the glass under normal room temperature conditions. Preparation of DNA Microarrays The microarrays were fabricated on poly-L-lysine coated microscope slides (Sigma). A custom-built arraying ma- chine, consist ing of four...
  9. ...-sgRNAs per gene) and synthesized on a microarray chip (Supplemental Table S1). These sgRNAs are designed to target 14,645 genes, and the distribution of these targeted genes across the is random (Supplemental Fig. S1), with these genes accounting for ∼86.76% of all genes in the . The rest of the genes, 13...
  10. ...Detecting genetic variation in microarray expression data Jennifer A. Greenhall 1 , 2 , 8 , Matthew A. Zapala 3 , 4 , 8 , Mario Cáceres 1 , 5 , Ondrej Libiger 4 , Carrolee Barlow 1 , 6 , Nicholas J. Schork 3 , 4...
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