Searching journal content for articles similar to Fernandez et al. 15 (2): 250.

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  1. ...26G-RNAs require Dicer but derive from an atypical RNA duplex and are produced exclusively antisense to their messenger RNA (mRNA) templates. To identify canonical siRNAs in C. elegans, we first characterized the siRNAs produced via the exogenous RNA interference (RNAi) pathway. During RNAi, ds...
  2. ...was previously validated by western blot analysis upon immunoprecipitation and RNAi depletion (Kranz et al. 2013). To confirm that ChIP-seq signal is dependent on PQN-85 protein, we degron-tagged pqn-85 endogenously and validated knockdown by western blot analysis (Supplemental Fig. S4A,B). Binding of PQN-85...
  3. ...CGC1, a new reference for Caenorhabditis elegans Kazuki Ichikawa1, Massa J. Shoura2,8, Karen L. Artiles2, Dae-Eun Jeong2, Chie Owa1, Haruka Kobayashi1, Yoshihiko Suzuki1, Manami Kanamori3, Yu Toyoshima3, Yuichi Iino3, Ann E. Rougvie4, Lamia Wahba5, Andrew Z. Fire2,6, Erich M. Schwarz7 and Shinichi...
  4. .... elegans embryonic cellsTo measure zygotic mRNA decay throughout C. elegans embryogenesis, we measured changes in gene expression after transcription inhibition (Fig. 1A). Primary cell cultures of C. elegans embryos divide and differentiate in ways that mirror development of intact embryos, with a similar...
  5. ...organism Encyclopedia of Regulatory Networks) consortia to systematically assay TF binding events in vivo in two major model organisms, Drosophila melanogaster (fly) and Caenorhabditis elegans (worm). These data sets comprise 605 TFs identifying 3.6 M sites in the fly and 356 TFs identifying 0.9 M sites...
  6. ...the presence of the 5′ adapter (Fig. 2B). To enhance the stability of mRNAs with exposed 5′ ends, we also performed a knockdown of the primary 5′→3′ exonuclease xrn-1 via RNAi (Supplemental Fig. S1A). The technique yields reproducible libraries (Supplemental Fig. S1B,C) of similar depth to each other...
  7. ...but has recently been found in a broad pattern in mouse oocytes and during aging in the C. elegans soma (Dahl et al. 2016; Pu et al. 2018). We therefore measured H3K4me3 peak breadth and found that H3K4me3 peaks were significantly broader in IGN than in embryos (Fig. 5D). Broad peaks (>3000 bp) were only...
  8. ...technology, such as inducible hairpin RNAi. Here we show that a C. elegans ORFeome resource, generated with the Gateway cloning system, can be used as a starting point to generate alternative HT-RNAi resources with enhanced flexibility. The versatility inherent to the Gateway system suggests that additional...
  9. ..., Canada; 4Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada Corresponding author: maja.tarailograovac@ucalgary.caAbstractGenetic balancers in Caenorhabditis elegans are complex variants that allow lethal or sterile mutations to be stably maintained...
  10. ...used high-throughput cDNA sequencing technologies. For example, in the annotation of the Caenorhabditis elegans transcriptome, more than half of the transcript isoforms lack full-length support and instead rely on inference from short reads that do not span the full length of the isoform. We applied...
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