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  1. ...2 : 7 . ↵ Eyras, E., Caccamo, M., Curwen, V., and Clamp, M. 2004 . ESTGenes: Alternative splicing from ESTs in Ensembl. Genome Res. (this issue). ↵ Karolchik, D., Hinrichs, A.S., Furey, T.S., Roskin, K.M., Sugnet, C.W., Haussler, D., and Kent, W.J. 2004 . The UCSC Table Browser data retrieval tool...
  2. ...) with parameters set such that the exon boundaries are not modified. The ESTs are useful in determining possible alternative splicing of the predicted genes ( Eyras et al. 2004 ). The methods used in predicting the Ensembl EST gene set are fully described elsewhere ( Eyras et al. 2004 ). Processed Pseudogene...
  3. ...), available as one of the gene prediction tracks in the UCSC browser, each node is the splice site and the edges are exons or introns connecting the splice sites. Each node in the ESTgenes of the Ensembl system represents the sequence itself, and two types of edge indicate the inclusion and extension...
  4. ...in the human . Nat. Genet. 29 : 412 –417. ↵ Eyras, E., Caccamo, M., Curwen, V., and Clamp, M. 2004 . ESTGenes: Alternative splicing from ESTs in Ensembl. Genome Res. 14 : 976 –987. ↵ Galante, P.A., Sakabe, N.J., Kirschbaum-Slager, N., and de Souza, S.J. 2004 . Detection and evaluation of intron retention...
  5. ...transcripts to improve those of higher confidence if necessary. The Gramene pipeline retains all high-confidence alternatively spliced transcripts based on the alignment score at the transcript level. In the original Ensembl pipeline, the number of predicted alternative splicing forms can become quite large...
  6. ...of interest along with all other annotations provided by Ensembl and VectorBase browsers (e.g., gene models, ESTs, transcripts). This feature can be used for confirming or altering the gene models of the existing annotations. Figure 1. Flowchart illustrating the proteogenomics analysis steps. Genome Research...
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