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  1. ...is recreated with the coordinates reversed for the second genomic sequence and the algorithm is run again to find the chains in the opposite orientation. Gregory et al. (2002) aligned mouse FPC contigs to the human minimal tiling path using mouse BESs, where they computed the anchors as BES matches with unique...
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  2. ..., R.A., and Bradley, A. 2001 . A clone-array pooled shotgun strategy for sequencing large s. Genome Res. 11 : 1619 -1623. ↵ Engler, F.W., Hatfield, J., Nelson, W., and Soderlund, C.A. 2003 . Locating sequence on FPC maps and selecting a minimal tiling path. Genome Res. 13 : 2152 -2163. ↵ Gordon, D...
  3. .... An * after a clone name indicates a parent clone. The clones highlighted in blue are the tiling path. The clone highlighted in cyan was selected with the mouse, which caused its markers and remarks to be highlighted in green and pink, respectively. The map units are the number of bands. New Features Recently...
  4. .....washington.edu/UWGC/analysistools/segmap.cfm ), and the clone-overgo information was imported into FPC . The resulting contigs were examined manually, and minimally overlapping sets of clones (sequence-tiling paths) were selected based on the fingerprint and overgo-content data. Updated sequence-tiling paths can be viewed at www.nisc.nih.gov . WEB SITE...
  5. ...were digested (on optical mapping surfaces) with restriction endonucleases, and images were collected using Gencol (see Methods). The basis of how shotgun optical mapping assembles whole maps is similar in many ways to random clone mapping approaches that assemble tiling paths across chromosomes...
  6. ...clone), then add those sizes to the cumulative size estimate; (3) repeat step 2 until all unique fragments in the tiling path clones have been identified and summed to give a total size estimate. Shared fragments are as defined by the FPC parameters used such that two fragments are considered the same...
  7. ...analyzed, as shown in Figure 1 A. Results indicate that the average insert size is 146 kb with >78% of the clones >100 kb. View larger version: In this window In a new window Figure 3. Six FPC contigs illustrated by a selected set of BAC clones providing a complete tiling path of the Bradyrhizobium...
  8. ...-six percent (51,935 tags) of the theoretically possible 60,676 unique in silico tags were also retrieved in the wet-lab WGP experiment (Table 1). Contig building using FPC and map quality assessment A cut-off value of 1.03 10#2;6 and a consecutiveDQ stepwas used in the FingerPrinted Contig (FPC) software...
  9. ...constructed using restriction enzyme digestion fingerprinting and specialized software ( Soderlund et al. 1997 ). A minimal tiling path is derived from the map, and each clone in the tiling path is shotgun-sequenced and assembled ( Kent and Haussler 2001 ; Choi and Farach-Colton 2003 ). The entire...
  10. ...25 canine BAC clones and 18 feline BAC clones (Pearks Wilkerson et al. 2008) selected from minimum tiling paths were sheared to ;3-kb insert length and pooled in equimolar ratios to prepare 454 Life Sciences (Roche) FLX paired-end libraries. Pooled libraries for dog and cat were each sequenced...
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