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ARTICLES: Ligation-mediated PCR of restriction fragments from large DNA molecules.
- D R Smith
Genome Res. August 1992 2: 21-27; doi:10.1101/gr.2.1.21
..., Inc., Waltham, Massachusetts 02154. Abstract A general method is described for PCR amplification of single restriction fragments from large DNA molecules. The method involves sequence-specific ligation of synthetic oligonucleotides to ambiguous 4-base 5' overhangs produced by type IIS restriction...
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GENOME METHODS: A Homogeneous, Ligase-Mediated DNA Diagnostic Test
- Xiangning Chen,
- Kenneth J. Livak,
- and Pui-Yan Kwok
Genome Res. May 1, 1998 8: 549-556; doi:10.1101/gr.8.5.549
...human DNA sequence variation. Science 278 : 1580 – 1581 . ↵ Couch F.J. , Weber B.L. ( 1996 ) Mutations and polymorphisms in the familial early-onset breast cancer (BRCA1) gene. Hum. Mutat. 8 : 8 – 18 . ↵ Eggerding F.A. ( 1995 ) A one-step coupled amplification and oligonucleotide ligation procedure...
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RESOURCE: Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications
- Pierre Hilson,
- Joke Allemeersch,
- Thomas Altmann,
- Sébastien Aubourg,
- Alexandra Avon,
- Jim Beynon,
- Rishikesh P. Bhalerao,
- Frédérique Bitton,
- Michel Caboche,
- Bernard Cannoot,
- Vasil Chardakov,
- Cécile Cognet-Holliger,
- Vincent Colot,
- Mark Crowe,
- Caroline Darimont,
- Steffen Durinck,
- Holger Eickhoff,
- Andéol Falcon de Longevialle,
- Edward E. Farmer,
- Murray Grant,
- Martin T.R. Kuiper,
- Hans Lehrach,
- Céline Léon,
- Antonio Leyva,
- Joakim Lundeberg,
- Claire Lurin,
- Yves Moreau,
- Wilfried Nietfeld,
- Javier Paz-Ares,
- Philippe Reymond,
- Pierre Rouzé,
- Goran Sandberg,
- Maria Dolores Segura,
- Carine Serizet,
- Alexandra Tabrett,
- Ludivine Taconnat,
- Vincent Thareau,
- Paul Van Hummelen,
- Steven Vercruysse,
- Marnik Vuylsteke,
- Magdalena Weingartner,
- Peter J. Weisbeek,
- Valtteri Wirta,
- Floyd R.A. Wittink,
- Marc Zabeau,
- and Ian Small
Genome Res. October 15, 2004 14: 2176-2189; doi:10.1101/gr.2544504
...repertoires (oligonucleotide, cDNA, or genomic DNA fragments) required for microarrays or RNAi is cumbersome and expensive. Ideally, this type of reference material should be formatted as robust and versatile resources, readily available to the research community. Using these criteria as a standard, we have...
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Method: Gene expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ
- Joseph W. Foley,
- Chunfang Zhu,
- Philippe Jolivet,
- Shirley X. Zhu,
- Peipei Lu,
- Michael J. Meaney,
- and Robert B. West
Genome Res. November 2019 29: 1816-1825; Published in Advance September 13, 2019, doi:10.1101/gr.234807.118
...version: In this window In a new window Figure 1. Conceptual diagram of the Smart-3SEQ library preparation method. Hands-on steps are separated by horizontal lines. (A) Total RNA is denatured and fragmented by hydrolysis. (B) The oligo(dT) primer, including a partial sequencing adapter, anneals...
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REVIEW: Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends
- Deirdre Meldrum
Genome Res. September 1, 2000 10: 1288-1303; doi:10.1101/gr.157400
...half of a two-part, noncomprehensive review that presents an overview of different types of automation equipment used in sequencing. The first part of the review, published in the previous issue, focused on automated procedures used to prepare DNA for sequencing or analysis. This second part...

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