Searching journal content for articles similar to Edmands et al. 3 (6): 317.

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  1. Method: Combining RT-PCR-seq and RNA-seq to catalog all genic elements encoded in the human genome
    • Cédric Howald,
    • Andrea Tanzer,
    • Jacqueline Chrast,
    • Felix Kokocinski,
    • Thomas Derrien,
    • Nathalie Walters,
    • Jose M. Gonzalez,
    • Adam Frankish,
    • Bronwen L. Aken,
    • Thibaut Hourlier,
    • Jan-Hinnerk Vogel,
    • Simon White,
    • Stephen Searle,
    • Jennifer Harrow,
    • Tim J. Hubbard,
    • Roderic Guigó,
    • and Alexandre Reymond
    Genome Res. September 2012 22: 1698-1710; doi:10.1101/gr.134478.111
    ...by RT-PCR amplification followed by highly multiplexed sequencing readout, a method we called RT-PCR-seq. Seventy-nine percent of all assessed junctions are confirmed by this evaluation procedure, demonstrating the high quality of the GENCODE gene set. RT-PCR-seq was also efficient to screen gene models...
    OPEN ACCESS ARTICLE
  2. Method: A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor
    • Hengxing Lu,
    • Jun Liu,
    • Tao Feng,
    • Zihang Guo,
    • Yunjun Yin,
    • Fei Gao,
    • Gengsheng Cao,
    • Xuguang Du,
    • and Sen Wu
    Genome Res. May 2021 31: 900-909; Published in Advance April 1, 2021, doi:10.1101/gr.271312.120
    ...-step gDNA removal and cDNA synthesis supermix (TransGen Biotech). RT-PCR was performed using green qPCR supermix (TransGen Biotech) and performed on an ABI7500 real-time PCR system. Primers used for RT-PCR are listed in Supplemental Table S7. Cultured cells were harvested and resuspended in RIPA lysis...
  3. Method: Rapid molecular assays to study human centromere genomics
    • Rafael Contreras-Galindo,
    • Sabrina Fischer,
    • Anjan K. Saha,
    • John D. Lundy,
    • Patrick W. Cervantes,
    • Mohamad Mourad,
    • Claire Wang,
    • Brian Qian,
    • Manhong Dai,
    • Fan Meng,
    • Arul Chinnaiyan,
    • Gilbert S. Omenn,
    • Mark H. Kaplan,
    • and David M. Markovitz
    Genome Res. December 2017 27: 2040-2049; Published in Advance November 15, 2017, doi:10.1101/gr.219709.116
    ...information on the DNA and RNA samples, analysis of centromere transcription by qRT-PCR, description of ChIP and IF-FISH experiments, amplification and sequencing analysis of K111, qPCR analysis to differentiate centromere 13 from centromere 21 using LNA technology, and NGS analysis of centromere sequences...
  4. REVIEW: Quantitative PCR and RT-PCR in virology.
    • M Clementi,
    • S Menzo,
    • P Bagnarelli,
    • A Manzin,
    • A Valenza,
    • and P E Varaldo
    Genome Res. February 1993 2: 191-196; doi:10.1101/gr.2.3.191
    ...using PCR and reverse transcrip- tion (RT)-PCR, and the applications of these methods in basic and diagnostic virology. THE IMPACT OF PCR AMPLIFICATION IN VIROLOGY Since the development of PCR amplifi- cation, one of the major applications of this method has been the highly sensi- tive and direct...
  5. ARTICLES: A novel method for real time quantitative RT-PCR.
    • U E Gibson,
    • C A Heid,
    • and P M Williams
    Genome Res. October 1996 6: 995-1001; doi:10.1101/gr.6.10.995
    ....D. and Larrick. J.W. 1992 . Competitive PCR. Nature 359 : 557 – 558 . Stoflet, E.S., Koeberl, D.D. Sarkar, G. and Sommer. S.S. 1988 . Genomic amplification with transcript sequencing. Science 239 : 491 – 494 . GENOME METHODS A Novel Method for Real Time Quantitative RT-PCR Ursula E.M. Gibson, 1 Christian A. Heid...
  6. Review: A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest
    • Andrew D. Bates,
    • Dawid Grzela,
    • Maciej Studzian,
    • Louise Brennan,
    • Moli Williams,
    • Conor Fawcett,
    • Beth Hammond,
    • Manreen Grewal,
    • Marcin Ratajewski,
    • Lukasz Pulaski,
    • and Urszula L. McClurg
    Genome Res. March 2026 36: 433-459; Published in Advance February 17, 2026, doi:10.1101/gr.281154.125
    ...within that droplet. The absolute number of target molecules is calculated using Poisson statistics, bypassing the need for standard curves and making ddPCR advantageous for low-abundance targets or small fold-changes that may fall below the detection limits of qRT-PCR. Because each amplification occurs...
    OPEN ACCESS ARTICLE
  7. ARTICLE: A method for rapid generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions.
    • R Ross,
    • R Kleiz,
    • and A B Reske-Kunz
    Genome Res. June 1995 4: 371-375
    ...generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions. A method for rapid generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions. R Ross , R Kleiz , and A B Reske-Kunz Department...
  8. ARTICLES: Modified tissue pulverization technique and evaluation of dihydrofolate reductase amplification as a pan-tissue RT PCR control.
    • P E Tam,
    • A M Schmidt,
    • and R P Messner
    Genome Res. August 1993 3: 71-72
    .... Menon, R.S., Chang, Y.F. St. Clair, J. and Ham. R.G. 1991 . RT-PCR artifacts from processed pseudogenes. PCR Methods Applic. 1 : 70 – 71 . lllllll Technical Tips Modified Tissue Pulverization Technique and Evaluation of Dihydrofolate Reductase Amplification as a Pan-tissue RT PCR Control Patricia E. Tam...
  9. ARTICLE: Accurate and absolute quantitative measurement of gene expression by single-tube RT-PCR and HPLC.
    • A Hayward-Lester,
    • P J Oefner,
    • S Sabatini,
    • and P A Doris
    Genome Res. December 1995 5: 494-499; doi:10.1101/gr.5.5.494
    ..., absolute quantification of gene expression in a single reverse transcriptase (RT)-PCR reaction. This method makes use of novel high-per- formance liquid chromatography (HPLC) technology to resolve and quantify the products of competi- tive, mutant RNA PCRs. The HPLC technique allows rapid, high resolu...
  10. ARTICLE: Quantitation of mRNA species by RT-PCR on total mRNA population.
    • S Hamoui,
    • J P Benedetto,
    • M Garret,
    • and J Bonnet
    Genome Res. December 1994 4: 160-166
    ...Quantitation of mRNA species by RT-PCR on total mRNA population. S Hamoui , J P Benedetto , M Garret , and J Bonnet Institut de Biochimie et de Génétique Cellulaires, Bordeaux, France. Abstract PCR is commonly used for m...
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