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REVIEW: On beyond classic RACE (rapid amplification of cDNA ends).
- M A Frohman
Genome Res. August 1994 4: S40-S58
...Brook, New York 11794-8651 Most attempts to identify and isolate a novel cDNA result in the acquisition of clones that represent only a part of the mRNA's complete sequence. The missing sequence (cDNA ends) can be cloned by PCR, using a technique variously called rapid amplification of cDNA ends (RACE...
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Method: Transcript amplification from single bacterium for transcriptome analysis
- Yun Kang,
- Michael H. Norris,
- Jan Zarzycki-Siek,
- William C. Nierman,
- Stuart P. Donachie,
- and Tung T. Hoang
Genome Res. June 2011 21: 925-935; Published in Advance May 2, 2011, doi:10.1101/gr.116103.110
...of membrane containing one or five fluorescent bacteriawere cut by the focused laser and catapulted with unfocused low-intensity laser beam into 2mL of the lysis buffer contained within a 0.2-mL PCR tube lid. The cDNA synthesis and amplification of the single cell total transcript were then performed as below...
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ARTICLE: Specific immunoglobulin cDNA clones produced from hybridoma cell lines and murine spleen fragment cultures by 3SR amplification.
- C A Stillman,
- P J Linton,
- P J Koutz,
- D J Decker,
- N R Klinman,
- and T R Gingeras
Genome Res. June 1994 3: 320-331
...-107/92-108 (leader region) and 92-109/92-110 (variable region) (Fig. 2A) were used with AMV RT to synthe- size the first strand of cDNA. Following the RT reaction, primer 92-140 (5'-ACT- A G AA TTCA G T GGA TA GA CA GA T GGGGG- TG-3'), which contains an inserted EcoRI site (italics), was used to complete the PCR...
ARTICLE: Construction of subtractive cDNA library using magnetic beads and PCR.
- A Lönneborg,
- P Sharma,
- and P Stougaard
Genome Res. February 1995 4: S168-S176
.... A rapid procedure for the construction of PCR cDNA libraries from small amounts of plant tissue. Plant Mol. Biol. Reporter 9 : 131 – 138 . 11.. Jacobsen, K.S., , Breivold, E. , Homes. E. Jacobsen, K.S., Breivold, E. and Homes. E. 1990 . Purification of mRNA directly from crude plant tissues in 15 minutes...
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ARTICLES: A simple PCR method for screening cDNA libraries.
- D Alfandari and
- T Darribère
Genome Res. August 1994 4: 46-49
...A (TER). Aliquots of these plasmid libraries were then used as templates for PCR to deter- mine the presence of the cDNA of inter- est and consequently to select the ph- agemid library to be screened. Titration of the Desired cDNA To evaluate the abundance of the desired cDNA clone in the selected...
ARTICLE: A rapid PCR protocol for identification of differentially expressed genes from a cDNA library.
- K J Mutchler,
- S W Klemish,
- and A F Russo
Genome Res. February 1992 1: 195-198; doi:10.1101/gr.1.3.195
...screenings and to facilitate the rapid screening of a relativelv large number of differentially expressed cDNA clones. To circumvent secondarv and ter- tiarv screenings of over 50 plaques showing differential hybridization, we PCR-amplified the various cDNA species directly from the eluted phage particles...
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METHODS: Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification
- Frank B. Dean,
- John R. Nelson,
- Theresa L. Giesler,
- and Roger S. Lasken
Genome Res. June 1, 2001 11: 1095-1099; doi:10.1101/gr.180501
...ET terminator reagent kit with a MegaBACE 1000. Multiply-primed RCA will have additional applications. With an error rate of 1 in 106–107 bases ( Esteban et al. 1993 ), φ29 DNA polymerase will be useful for amplification of genomic DNA or cDNA. Amplification of genomic DNA may require some additional...
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METHODS: Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes
- Piero Carninci,
- Yuko Shibata,
- Norihito Hayatsu,
- Yuichi Sugahara,
- Kazuhiro Shibata,
- Masayoshi Itoh,
- Hideaki Konno,
- Yasushi Okazaki,
- Masami Muramatsu,
- and Yoshihide Hayashizaki
Genome Res. October 1, 2000 10: 1617-1630; doi:10.1101/gr.145100
...to normalize and subtract cDNA before cloning. Published protocols did not lead to equal representation among clones of different sizes, maintain the length of long cDNAs after hybridization, or incorporate simultaneous normalization and subtraction of cDNAs. Therefore, methods based on PCR ( Takahashi and Ko...
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ARTICLE: Targeting a Complex Transcriptome: The Construction of the Mouse Full-Length cDNA Encyclopedia
- Piero Carninci,
- Kazunori Waki,
- Toshiyuki Shiraki,
- Hideaki Konno,
- Kazuhiro Shibata,
- Masayoshi Itoh,
- Katsunori Aizawa,
- Takahiro Arakawa,
- Yoshiyuki Ishii,
- Daisuke Sasaki,
- Hidemasa Bono,
- Shinji Kondo,
- Yuichi Sugahara,
- Rintaro Saito,
- Naoki Osato,
- Shiro Fukuda,
- Kenjiro Sato,
- Akira Watahiki,
- Tomoko Hirozane-Kishikawa,
- Mari Nakamura,
- Yuko Shibata,
- Ayako Yasunishi,
- Noriko Kikuchi,
- Atsushi Yoshiki,
- Moriaki Kusakabe,
- Stefano Gustincich,
- Kirk Beisel,
- William Pavan,
- Vassilis Aidinis,
- Akira Nakagawara,
- William A. Held,
- Hiroo Iwata,
- Tomohiro Kono,
- Hiromitsu Nakauchi,
- Paul Lyons,
- Christine Wells,
- David A. Hume,
- Michela Fagiolini,
- Takao K. Hensch,
- Michelle Brinkmeier,
- Sally Camper,
- Junji Hirota,
- Peter Mombaerts,
- Masami Muramatsu,
- Yasushi Okazaki,
- Jun Kawai,
- and Yoshihide Hayashizaki
Genome Res. June 2003 13: 1273-1289; doi:10.1101/gr.1119703
.... Microdissection We prepared full-length libraries from small tissues, including 71 microdissected tissues (Tables 1 and 3 ). To this end, we modified the Cap-Trapper for small quantities of total RNA, without PCR, which might skew representation, especially at expanses of long cDNAs and would affect...
ARTICLE: Panhandle PCR.
- D H Jones
Genome Res. April 1995 4: S195-S201
...tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification. Nucleic Acids Res. 19: 5227-5232. 9. Troutt, A.B., M.G. McHeyzer-Williams, B. Pulendran, and G.J.V. Nossal. 1992. Ligation-an- chored PCR: A simple amplification technique with single-sided specificity...
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