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Research: Two contrasting classes of nucleolus-associated domains in mouse fibroblast heterochromatin
- Anastassiia Vertii,
- Jianhong Ou,
- Jun Yu,
- Aimin Yan,
- Hervé Pagès,
- Haibo Liu,
- Lihua Julie Zhu,
- and Paul D. Kaufman
Genome Res. August 2019 29: 1235-1249; Published in Advance June 14, 2019, doi:10.1101/gr.247072.118
...is developmentally regulated, and present in MEFs but not in undifferentiated embryonic stem (ES) cells.As the site of ribosome biogenesis, nucleoli are organized around rDNA repeats (McStay and Grummt 2008; Pederson 2011) and consist of liquid-phase separated and functionally distinct layers of proteins and RNAs...
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Research: The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity
- Kristina Ivanauskiene,
- Erwan Delbarre,
- James D. McGhie,
- Thomas Küntziger,
- Lee H. Wong,
- and Philippe Collas
Genome Res. October 2014 24: 1584-1594; Published in Advance July 21, 2014, doi:10.1101/gr.173831.114
...studies show that nonnucleosomal H3.3 normally destined to PML nuclear bodies is re-routed to chromatin after depletion of DEK. This results in HIRA-dependent widespread chromatin deposition of H3.3 and H3.3 incorporation in the foci of heterochromatin in a process requiring the DAXX/ATRX complex...

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