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Research: PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX
- Erwan Delbarre,
- Kristina Ivanauskiene,
- Jane Spirkoski,
- Akshay Shah,
- Kristin Vekterud,
- Jan Øivind Moskaug,
- Stig Ove Bøe,
- Lee H. Wong,
- Thomas Küntziger,
- and Philippe Collas
Genome Res. June 2017 27: 913-921; Published in Advance March 24, 2017, doi:10.1101/gr.215830.116
...environment of histone variant H3.3 revealed by quantitative imaging and -scale chromatin and DNA immunoprecipitation. Mol Biol Cell 21: 1872–1884. Delbarre E, Ivanauskiene K, Kuntziger T, Collas P. 2013. DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending deposition into chromatin. Genome...
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Research: The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity
- Kristina Ivanauskiene,
- Erwan Delbarre,
- James D. McGhie,
- Thomas Küntziger,
- Lee H. Wong,
- and Philippe Collas
Genome Res. October 2014 24: 1584-1594; Published in Advance July 21, 2014, doi:10.1101/gr.173831.114
...and DAPI-dense marks. Bars, 10 mm. Ivanauskiene et al. 1586 Genome Research www..org We infer from these data either that DEK interferes with the DAXX-dependent targeting of H3.3 to PML NBs, or that DEK promotes the maintenance of a soluble pool of H3.3, perhaps by configuring chromatin in a way...

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