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  1. ...,000 generations in the fission yeast Schizosaccharomyces pombe (Farlow et al. 2015).We calculated the mean neutrality index (NI) NITG (Stoletzki and Eyre-Walker 2011) for each lineage using polymorphism data from each lineage and S. paradoxus as an outgroup (Fig. 5A). NITG was higher in the domesticated lineages...
  2. ...-Perez, I., and Nurse, P. 2003 . Schizosaccharomyces pombe essential genes: A pilot study. Genome Res. 13 : 399 -406. ↵ Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M., and Ahringer, J. 2000 . Functional genomic analysis of C. elegans chromosome I by systematic RNA interference...
  3. ...contributes to the completion of DNA replication; and the relationship of origins with nuclear replication foci is unclear. Here we address these questions in the fission yeast Schizosaccharomyces pombe by analyzing long single DNA molecules that can approach the full length of chromosomes. The fission yeast...
  4. ...), the numbers of TSS supported by at least two tags are 227,021 and 204,197, respectively (Supplemental Fig. S2). TSS-seq data mapped 225,563 TSSs supported by TPM ≥ 0.1 (Malabat et al. 2015). In the fission yeast Schizosaccharomyces pombe, which has a similar size (12.61 Mb) as S. cerevisiae, only 93,736 CTSSs...
  5. ...the Fosill strategy, we constructed a Fosmid library from 30 mg Schizosaccharomyces pombe genomic DNA. The library size, as estimated by plating smallscale test transductions on chloramphenicol plates, was 1.4million colony-forming units (cfu). We performed a large-scale transduction with the remainder...
  6. ...2000 ). In fungal species they account for roughly one fifth of the total genes: 17.8% in Saccharomyces cerevisiae ( Winzeler et al. 1999 ; Giaever et al. 2002 ), and 17.5% of the small subset in Schizosaccharomyces pombe studied to date ( Decottignies et al. 2003 ). Because phenotype is a product...
  7. ...–484. Dai J, Chuang RY, Kelly TJ. 2005. DNA replication origins in the Schizosaccharomyces pombe . Proc Natl Acad Sci 102: 337–342. Delgado S, Go´mez M, Bird A, Antequera F. 1998. Initiation of DNA replication at CpG islands in mammalian chromosomes. EMBO J 17: 2426–2435. Desprat R, Thierry-Mieg D, Lailler...
  8. ...; Pruitt et al. 2005 ). We determined the percentage of annotated small proteins from additional eukaryotes in the NCBI RefSeq database (see Methods). We selected representative eukaryotes including another fungus ( Schizosaccharomyces pombe ), worms ( Caenorhabditis elegans ), plants ( Arabidopsis...
  9. ...elements in 1% of the human by the ENCODE pilot project . Nature , (in press) . ↵ Euskirchen, G. , Royce, T.E. , Bertone, P. , Martone, R. , Rinn, J.L. , Nelson, F.K. , Sayward, F. , Luscombe, N.M. , Miller, P. , Gerstein, M. , et al. ( 2004 ) CREB binds to multiple loci on human chromosome 22. Mol. Cell...
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