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Method: Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining
- Marcello Maresca,
- Victor Guosheng Lin,
- Ning Guo,
- and Yi Yang
Genome Res. March 2013 23: 539-546; Published in Advance November 14, 2012, doi:10.1101/gr.145441.112
..., Miller JC, et al. 2010. Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human . Genome Res 20: 1133–1142. Doyon Y, Vo TD, Mendel MC, Greenberg SG, Wang J, Xia DF, Miller JC, Gregory PD, Holmes...
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Research: Systematic interrogation of human promoters
- Shira Weingarten-Gabbay,
- Ronit Nir,
- Shai Lubliner,
- Eilon Sharon,
- Yael Kalma,
- Adina Weinberger,
- and Eran Segal
Genome Res. February 2019 29: 171-183; Published in Advance January 8, 2019, doi:10.1101/gr.236075.118
...ssDNA oligos 200 nt in length on Agilent programmable arrays and harvested them as a single pool. Oligos were amplified by PCR using constant primers and cloned into pZDonor plasmid upstream of eGFP. The plasmid pool was conucleofected with mRNAs encoding zinc finger nucleases (ZFNs) targeting...

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