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Research: Adaptation of centromeres to breakage through local genomic and epigenomic remodeling in wheat
- Jingwei Zhou,
- Yuhong Huang,
- Huan Ma,
- Yiqian Chen,
- Chuanye Chen,
- Fangpu Han,
- and Handong Su
Genome Res. November 2025 35: 2461-2471; Published in Advance September 30, 2025, doi:10.1101/gr.280913.125
...fragments. Studies using CRISPR-Cas9-induced centromeric DSBs in mammalian cells have shown activation of either homologous recombination (HR) or nonhomologous end joining (NHEJ) pathways (Tsouroula et al. 2016; Saayman et al. 2023). DNA-end resection, facilitated by the formation of DNA–RNA hybrids (R...
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Perspective: Enabling functional genomics with genome engineering
- Isaac B. Hilton and
- Charles A. Gersbach
Genome Res. October 2015 25: 1442-1455; doi:10.1101/gr.190124.115
...exogenous DNA at genomic loci (Smithies et al. 1985; Thomas et al. 1986). The efficiency of this method is dramatically enhanced in the presence of double-strand breaks (DSBs) (Rouet et al. 1994). Cells generally use two distinct pathways to resolve DSBs (Chapman et al. 2012): homology-directed repair (HDR...
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