Searching journal content for articles similar to Craig et al. 19 (11): 2075.

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  1. ...A large-scale zebrafish gene knockout resource for the genome-wide study of gene function Gaurav K. Varshney 1 , 8 , Jing Lu 2 , 3 , 8 , Derek E. Gildea 4 , Haigen Huang 3 , Wuhong Pei 1 , Zhongan Yang 3 , Sunny C. Huang...
  2. ...organisms, such as Drosophila and Caenorhabditis elegans, that are amenable to both forward genetic screens and pooled RNA-seq experiments. Thus, MMAPPR is a rapid, cost-efficient, and highly automated pipeline, available to perform mutant mapping in any organism with a well-assembled . [Supplemental...
  3. ...hypersensitivity), high nucleosomal turnover (e.g., high levels of rapid-turnover histones H2A.Z and H3.3), specific epigenetic markers (e.g., high ratio of H3K4me1 and H3K4me2 to H3K4me3, high ratio of 5hmC to 5mC, enrichment of H3K27ac), chromatin loops in direct contact with the core promoter (enrichment...
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  4. ...even when s are ‘‘assembled’’ by alignment to a known sequence. For example, the D. simulans project involved placement of most contigs onto Figure 2. Genome-wide ancestry assignment for a representative individual. (A) The ancestry states are shown for all major chromosome arms for a representative...
  5. ...of SAMtools and BCFtools. GigaScience 10: giab008. doi:10.1093/gigascience/giab008 ↵Davey JW, Hohenlohe PA, Etter PD, Boone JQ, Catchen JM, Blaxter ML. 2011. Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nat Rev Genet 12: 499–510. doi:10.1038/nrg3012 ↵Deng T, Zhang P...
  6. ...R estriction site a ssociated D NA (RAD) tags are a genome-wide representation of every site of a particular restriction enzyme by short DNA tags. Most organisms segregate large numbers of DNA sequence polymorphisms that disrupt restriction sites, which allows RAD tags to serve as genetic markers...
  7. ...-BAGs). Unlike other “cell bead” ideas (Tamminen and Virta 2015; Andor et al. 2018), in C-BAGs the first copy of nuclei acids is covalently bound to the gel matrix and individually tagged by a split-and-pool strategy.The apparatus is inexpensive, and we estimate the cost per cell to be $0.50 when performed...
  8. ...is of less value than a rapid workflow.The removal of Tn5 effectively releases the two end fragments from one another that were generated during the reaction, each receiving one of the adapters from the transposome complex and retaining one strand of the 9-bp region in between the two cut sites. Extension...
  9. ....Recent advances in next-generation sequencing (NGS) technologies now allow rapid and affordable generation of extensive genomic resources in numerous less-studied organisms and offer opportunities to address many scientific questions with unprecedented power and precision (Levy and Myers 2016). During the last...
  10. ...are large changes in chromatin accessibility, which then induces subsequent gene expression changes in the time course.View larger version: In this window In a new window Figure 2. Genome-wide profiling of gene expression and chromatin accessibility during RA induction reveals landscape for RA...
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