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Craig et al. 19 (11): 2075
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26
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:
A large-scale zebrafish gene knockout resource for the genome-wide study of gene function
Gaurav K. Varshney
,
Jing Lu
,
Derek E. Gildea
,
Haigen Huang
,
Wuhong Pei
,
Zhongan Yang
,
Sunny C. Huang
,
David Schoenfeld
,
Nam H. Pho
,
David Casero
,
Takashi Hirase
,
Deborah Mosbrook-Davis
,
Suiyuan Zhang
,
Li-En Jao
,
Bo Zhang
,
Ian G. Woods
,
Steven Zimmerman
,
Alexander F. Schier
,
Tyra G. Wolfsberg
,
Matteo Pellegrini
,
Shawn M. Burgess
,
and
Shuo Lin
Genome Res.
April 2013
23
:
727
-
735
;
Published in Advance
February 4, 2013
,
doi:
10.1101/gr.151464.112
...A large-scale zebrafish gene knockout resource for the
genome-wide
study of gene function Gaurav K. Varshney 1 , 8 , Jing Lu 2 , 3 , 8 , Derek E. Gildea 4 , Haigen Huang 3 , Wuhong Pei 1 , Zhongan Yang 3 , Sunny C. Huang...
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Method
:
MMAPPR: Mutation Mapping Analysis Pipeline for Pooled RNA-seq
Jonathon T. Hill
,
Bradley L. Demarest
,
Brent W. Bisgrove
,
Bushra Gorsi
,
Yi-Chu Su
,
and
H. Joseph Yost
Genome Res.
April 2013
23
:
687
-
697
;
Published in Advance
January 8, 2013
,
doi:
10.1101/gr.146936.112
...organisms, such as Drosophila and Caenorhabditis elegans, that are amenable to both forward genetic screens and
pooled
RNA-seq experiments. Thus, MMAPPR is a
rapid
, cost-efficient, and highly automated pipeline, available to perform mutant mapping in any organism with a well-assembled . [Supplemental...
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Review
:
A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest
Andrew D. Bates
,
Dawid Grzela
,
Maciej Studzian
,
Louise Brennan
,
Moli Williams
,
Conor Fawcett
,
Beth Hammond
,
Manreen Grewal
,
Marcin Ratajewski
,
Lukasz Pulaski
,
and
Urszula L. McClurg
Genome Res.
March 2026
36
:
433
-
459
;
Published in Advance
February 17, 2026
,
doi:
10.1101/gr.281154.125
...hypersensitivity), high nucleosomal turnover (e.g., high levels of
rapid
-turnover histones H2A.Z and H3.3), specific epigenetic markers (e.g., high ratio of H3K4me1 and H3K4me2 to H3K4me3, high ratio of 5hmC to 5mC, enrichment of H3K27ac), chromatin loops in direct contact with the core promoter (enrichment...
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Method
:
Multiplexed shotgun genotyping for rapid and efficient genetic mapping
Peter Andolfatto
,
Dan Davison
,
Deniz Erezyilmaz
,
Tina T. Hu
,
Joshua Mast
,
Tomoko Sunayama-Morita
,
and
David L. Stern
Genome Res.
April 2011
21
:
610
-
617
;
Published in Advance
January 13, 2011
,
doi:
10.1101/gr.115402.110
...even when s are ‘‘assembled’’ by alignment to a known sequence. For example, the D. simulans project involved placement of most contigs onto Figure 2.
Genome-wide
ancestry assignment for a representative individual. (A) The ancestry states are shown for all major chromosome arms for a representative...
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Method
:
CRISPR-Cas9-based repeat depletion for high-throughput genotyping of complex plant genomes
Marzia Rossato
,
Luca Marcolungo
,
Luca De Antoni
,
Giulia Lopatriello
,
Elisa Bellucci
,
Gaia Cortinovis
,
Giulia Frascarelli
,
Laura Nanni
,
Elena Bitocchi
,
Valerio Di Vittori
,
Leonardo Vincenzi
,
Filippo Lucchini
,
Kirstin E. Bett
,
Larissa Ramsay
,
David James Konkin
,
Massimo Delledonne
,
and
Roberto Papa
Genome Res.
May 2023
33
:
787
-
797
;
Published in Advance
May 1, 2023
,
doi:
10.1101/gr.277628.122
...of SAMtools and BCFtools. GigaScience 10: giab008. doi:10.1093/gigascience/giab008 ↵Davey JW, Hohenlohe PA, Etter PD, Boone JQ, Catchen JM, Blaxter ML. 2011.
Genome-wide
genetic marker discovery and genotyping using next-generation sequencing. Nat Rev Genet 12: 499–510. doi:10.1038/nrg3012 ↵Deng T, Zhang P...
Abstract
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Methods
:
Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers
Michael R. Miller
,
Joseph P. Dunham
,
Angel Amores
,
William A. Cresko
,
and
Eric A. Johnson
Genome Res.
February 2007
17
:
240
-
248
;
Published in Advance
December 22, 2006
,
doi:
10.1101/gr.5681207
...R estriction site a ssociated D NA (RAD) tags are a
genome-wide
representation of every site of a particular restriction enzyme by short DNA tags. Most organisms segregate large numbers of DNA sequence polymorphisms that disrupt restriction sites, which allows RAD tags to serve as genetic markers...
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Method
:
Copolymerization of single-cell nucleic acids into balls of acrylamide gel
Siran Li
,
Jude Kendall
,
Sarah Park
,
Zihua Wang
,
Joan Alexander
,
Andrea Moffitt
,
Nissim Ranade
,
Cassidy Danyko
,
Bruno Gegenhuber
,
Stephan Fischer
,
Brian D. Robinson
,
Herbert Lepor
,
Jessica Tollkuhn
,
Jesse Gillis
,
Eric Brouzes
,
Alex Krasnitz
,
Dan Levy
,
and
Michael Wigler
Genome Res.
January 2020
30
:
49
-
61
;
Published in Advance
November 14, 2019
,
doi:
10.1101/gr.253047.119
...-BAGs). Unlike other “cell bead” ideas (Tamminen and Virta 2015; Andor et al. 2018), in C-BAGs the first copy of nuclei acids is covalently bound to the gel matrix and individually tagged by a split-and-
pool
strategy.The apparatus is
inexpensive
, and we estimate the cost per cell to be $0.50 when performed...
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Perspective
:
Tagmentation-based single-cell genomics
Andrew C. Adey
Genome Res.
October 2021
31
:
1693
-
1705
;
doi:
10.1101/gr.275223.121
...is of less value than a
rapid
workflow.The removal of Tn5 effectively releases the two end fragments from one another that were generated during the reaction, each receiving one of the adapters from the transposome complex and retaining one strand of the 9-bp region in between the two cut sites. Extension...
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Method
:
HD-Marker: a highly multiplexed and flexible approach for targeted genotyping of more than 10,000 genes in a single-tube assay
Jia Lv
,
Wenqian Jiao
,
Haobing Guo
,
Pingping Liu
,
Ruijia Wang
,
Lingling Zhang
,
Qifan Zeng
,
Xiaoli Hu
,
Zhenmin Bao
,
and
Shi Wang
Genome Res.
December 2018
28
:
1919
-
1930
;
Published in Advance
November 8, 2018
,
doi:
10.1101/gr.235820.118
....Recent advances in next-generation sequencing (NGS) technologies now allow
rapid
and affordable generation of extensive genomic resources in numerous less-studied organisms and offer opportunities to address many scientific questions with unprecedented power and precision (Levy and Myers 2016). During the last...
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Method
:
Time course regulatory analysis based on paired expression and chromatin accessibility data
Zhana Duren
,
Xi Chen
,
Jingxue Xin
,
Yong Wang
,
and
Wing Hung Wong
Genome Res.
April 2020
30
:
622
-
634
;
Published in Advance
March 18, 2020
,
doi:
10.1101/gr.257063.119
...are large changes in chromatin accessibility, which then induces subsequent gene expression changes in the time course.View larger version: In this window In a new window Figure 2.
Genome-wide
profiling of gene expression and chromatin accessibility during RA induction reveals landscape for RA...
Abstract
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