Searching journal content for articles similar to Cotta-de-Almeida et al. 13 (9): 2190.

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  1. METHODS: Highly Efficient Modification of Bacterial Artificial Chromosomes (BACs) Using Novel Shuttle Vectors Containing the R6Kγ Origin of Replication
    • Shiaoching Gong,
    • Xiangdong William Yang,
    • Chenjian Li,
    • and Nathaniel Heintz
    Genome Res. December 1, 2002 12: 1992-1998; doi:10.1101/gr.476202
    ...could confer very lower background. We hypothesized that a shuttle vector containing the R6kγ origin, the RecA gene and a recombination cassette (Fig. 1 A), when transformed into BAC host bacteria, may express sufficient RecA protein to support homologous recombination between the shuttle vector...
  2. METHODS: A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
    • Pentao Liu,
    • Nancy A. Jenkins,
    • and Neal G. Copeland
    Genome Res. March 1, 2003 13: 476-484; doi:10.1101/gr.749203
    ...a targeting vector. Originally, recombineering was done in yeast ( Baudin et al. 1993 ). However, during the past couple of years, it has also become possible to perform recombineering in Escherichia coli . Bacteria offer a number of advantages over yeast for recombineering. For example, manipulating...
  3. Methods: Rapid identification of homologous recombinants and determination of gene copy number with reference/query pyrosequencing (RQPS)
    • Zhenyi Liu,
    • Anna C. Obenauf,
    • Michael R. Speicher,
    • and Raphael Kopan
    Genome Res. November 2009 19: 2081-2089; Published in Advance October 1, 2009, doi:10.1101/gr.093856.109
    .... 1995. Notch1 is required for the coordinate segmentation of somites. Development 121: 1533–1545. Cotta-de-Almeida V, Schonhoff S, Shibata T, Leiter A, Snapper SB. 2003. A new method for rapidly generating gene-targeting vectors by engineering BACs through homologous recombination in bacteria. Genome...
  4. Method: Seamless gene correction of β-thalassemia mutations in patient-specific iPSCs using CRISPR/Cas9 and piggyBac
    • Fei Xie,
    • Lin Ye,
    • Judy C. Chang,
    • Ashley I. Beyer,
    • Jiaming Wang,
    • Marcus O. Muench,
    • and Yuet Wai Kan
    Genome Res. September 2014 24: 1526-1533; Published in Advance August 5, 2014, doi:10.1101/gr.173427.114
    .... CRISPR/Cas system and its role in phage-bacteria interactions. Annu Rev Microbiol 64: 475–493. Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, Bernstein ID, Collins JJ, Zon LI, et al. 2013. Induction of multipotential hematopoietic progenitors from human pluripotent stem cells via...
  5. Method: Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling
    • Yo Suzuki,
    • Nacyra Assad-Garcia,
    • Maxim Kostylev,
    • Vladimir N. Noskov,
    • Kim S. Wise,
    • Bogumil J. Karas,
    • Jason Stam,
    • Michael G. Montague,
    • Timothy J. Hanly,
    • Nico J. Enriquez,
    • Adi Ramon,
    • Gregory M. Goldgof,
    • R. Alexander Richter,
    • Sanjay Vashee,
    • Ray-Yuan Chuang,
    • Elizabeth A. Winzeler,
    • Clyde A. Hutchison III,
    • Daniel G. Gibson,
    • Hamilton O. Smith,
    • John I. Glass,
    • and J. Craig Venter
    Genome Res. March 2015 25: 435-444; Published in Advance February 5, 2015, doi:10.1101/gr.182477.114
    ..., La Jolla, California 92093, USA Corresponding author: ysuzuki@jcvi.org Abstract The availability of genetically tractable organisms with simple genomes is critical for the rapid, systems-level understanding of basic biological processes. Mycoplasma bacteria, with the smallest...
  6. PERSPECTIVE: Genomics of the future: Identification of quantitative trait loci in the mouse
    • Lorraine Flaherty,
    • Bruce Herron,
    • and Derek Symula
    Genome Res. December 2005 15: 1741-1745; doi:10.1101/gr.3841405
    ...for mouse functional genomics. Nat. Rev. Genet. 2 : 769 -779. ↵ Cotta-de-Almeida, V., Schonhoff, S., Shibata, T., Leiter, A., and Snapper, S.B. 2003 . A new method for rapidly generating gene-targeting vectors by engineering BACs through homologous recombination in bacteria. Genome Res. 13 : 2190 -2194...
  7. Method: Transcript amplification from single bacterium for transcriptome analysis
    • Yun Kang,
    • Michael H. Norris,
    • Jan Zarzycki-Siek,
    • William C. Nierman,
    • Stuart P. Donachie,
    • and Tung T. Hoang
    Genome Res. June 2011 21: 925-935; Published in Advance May 2, 2011, doi:10.1101/gr.116103.110
    .... GSE23419. The sequencing data have been submitted to GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) under accession nos. JG731867–JG732058.] Innovative methods in single-cell technology are needed to enhance the investigations of Bacteria and Archaea genomic material, particularly if we are to develop...
  8. REVIEW: Functional Genomics in the Mouse: Phenotype-Based Mutagenesis Screens
    • John Schimenti and
    • Maja Bucan
    Genome Res. July 1, 1998 8: 698-710; doi:10.1101/gr.8.7.698
    ...). Similarly, the application of gene traps to ES cells has enabled insertional mutagenesis akin to transposon tagging in flies, bacteria, and plants. These approaches have endowed the mouse with a gene-based means of mutagenesis—that is, it has become relatively simple to generate a mutation of any known...
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