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  1. ..., StempleDL. 2012. Incorporating RNA-seq data into the zebrafish Ensembl genebuild. Genome Res 22: 2067–2078. Cristea S, Freyvert Y, Santiago Y, Holmes MC, Urnov FD, Gregory PD, Cost GJ. 2013. In vivo cleavage of transgene donors promotes nucleasemediated targeted integration. Biotechnol Bioeng 110: 871...
  2. .... To that end, we performed ChIP-seq experiments with specific antibodies against H3K4me3 (histone 3 lysine 4 trimethylation) and H3K27ac (Fig. 3A). The reads obtained from sequencing of immunoprecipitated DNA were aligned to the medaka (oryLat2 assembly, Ensembl) (Flicek et al. 2013). Then, we used the H3K4me3...
  3. ...RNAs (ncRNAs) were annotated using the method adapted from Ensembl (http://useast.ensembl.org/info//genebuild/ncrna.html).Details of the procedures are provided in Supplemental Methods (Genome annotation).Orthology assignmentOrthology of genes was assigned by a clustering of sequence similarity...
  4. ...rabbit (oryCun2), Egyptian jerboa (jacJac1), and human (hg38) as outgroups. To provide hints to AUGUSTUS, RNA-seq data were obtained from the NCBI Sequence Read Archive (SRA) (Supplemental Table S2; Cortez et al. 2014; Fushan et al. 2015; Liu et al. 2016). For comparison we used existing Ensembl and Ref...
  5. ...(the gene set of the ENCODE project [The ENCODE Project Consortium 2012]) represents a merge between manually annotated HAVANA and computationally derived Ensembl models, with annotation taking place on the sequence. In contrast, while RefSeq also combines manual and automated processes, most human...
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  6. ...conducted on a simulated 35-bp RNA-seq data set based on the set of zebrafish cDNA from Ensembl (Supplemental Table S1). As expected, the average coverage of assembled contigs increases with increasing k-mer values on both the real (Table 2) and the simulated (Supplemental Table S2) data sets. However...
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