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LETTER: 15,000 Unique Zebrafish EST Clusters and Their Future Use in Microarray for Profiling Gene Expression Patterns During Embryogenesis
- Jane Lo,
- Sorcheng Lee,
- Min Xu,
- Feng Liu,
- Hua Ruan,
- Alvin Eun,
- Yawen He,
- Weiping Ma,
- Weefuen Wang,
- Zilong Wen,
- and Jinrong Peng
Genome Res. March 1, 2003 13: 455-466; doi:10.1101/gr.885403
...Immunology Lab, Institute of Molecular and Cell Biology, Singapore 117609 Abstract A total of 15,590 unique zebrafish EST clusters from two cDNA libraries have been identified. Most significantly, only 22% (3437) of the 15,590 unique clusters matched 2805 (of 15,200) clusters in the Danio rerio...
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Methods: Large-scale screening for novel low-affinity extracellular protein interactions
- K. Mark Bushell,
- Christian Söllner,
- Benjamin Schuster-Boeckler,
- Alex Bateman,
- and Gavin J. Wright
Genome Res. April 2008 18: 622-630; Published in Advance February 22, 2008, doi:10.1101/gr.7187808
...) and amplified by RT-PCR from mixed-stage zebrafish cDNA; (2) zebrafish IMAGE clones (85 genes) that were predicted to encode IgSF domains and a complete signal peptide were identified from EST databases by TBLASTN searches using all human and mouse IgSF-domain-encoding protein sequences; and (3) novel genes...
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