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LETTER: The Zebrafish Annexin Gene Family
- Steven A. Farber,
- Robert A. De Rose,
- Eric S. Olson,
- and Marnie E. Halpern
Genome Res. June 2003 13: 1082-1096; doi:10.1101/gr.479603
...function, we have cloned 11 zebrafish anx genes. Four genes ( anx1a, anx2a, anx5 ,and anx11a) were identified by screening a zebrafish cDNA library with a Xenopus anx2 fragment. For these genes, full-length cDNA sequences were used to cluster 212 EST sequences generated by the Zebrafish Genome...
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RESOURCE: The LN54 Radiation Hybrid Map of Zebrafish Expressed Sequences
- Neil Hukriede,
- Dan Fisher,
- Jonathan Epstein,
- Lucille Joly,
- Patricia Tellis,
- Yi Zhou,
- Brad Barbazuk,
- Kristine Cox,
- Laura Fenton-Noriega,
- Candace Hersey,
- Jennifer Miles,
- Xiaoming Sheng,
- Anhua Song,
- Rick Waterman,
- Stephen L. Johnson,
- Igor B. Dawid,
- Mario Chevrette,
- Leonard I. Zon,
- John McPherson,
- and Marc Ekker
Genome Res. December 1, 2001 11: 2127-2132; Published in Advance November 12, 2001, doi:10.1101/gr.210601
....W. , Marra M.A. , Lehrach H. , Johnson S.L. ( 2001 ) An oligonucleotide fingerprint normalized and EST characterized zebrafish cDNA library. Genome Res. 11 : 1594 – 1602 . ↵ Daga R.R. , Thode G. , Amores A. ( 1996 ) Chromosome complement, C-banding, Ag-NOR and replication banding in the zebrafish Danio rerio...
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REVIEW: Zebrafish: Genomics on the Fast Track
- David R. Beier
Genome Res. January 1, 1998 8: 9-17; doi:10.1101/gr.8.1.9
...at Washington University is available at http://.wustl.edu/est/zebrafish_esthmpg.html . A complement to EST characterization is the generation of arrayed cDNA libraries that can be probed by hybridization to identify zebrafish homologs of genes cloned from other species. By hybridization of murine Hox and Pax...
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LETTER: Expression Profiling and Comparative Genomics Identify a Conserved Regulatory Region Controlling Midline Expression in the Zebrafish Embryo
- Thomas Dickmeis,
- Charles Plessy,
- Sepand Rastegar,
- Pia Aanstad,
- Ralf Herwig,
- Frédéric Chalmel,
- Nadine Fischer,
- and Uwe Strähle
Genome Res. February 2004 14: 228-238; Published in Advance January 12, 2004, doi:10.1101/gr.1819204
.... Construction and analysis of arrayed cDNA libraries. Methods Enzymol. 303 : 205 -233. ↵ Clark, M.D., Hennig, S., Herwig, R., Clifton, S.W., Marra, M.A., Lehrach, H., Johnson, S.L., and the WU-GSC EST Group. 2001 . An oligonucleotide fingerprint normalized and expressed sequence tag characterized zebrafish cDNA...
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RESOURCE: Generation, Annotation, Evolutionary Analysis, and Database Integration of 20,000 Unique Sea Urchin EST Clusters
- Albert J. Poustka,
- Detlef Groth,
- Steffen Hennig,
- Sabine Thamm,
- Andrew Cameron,
- Alfred Beck,
- Richard Reinhardt,
- Ralf Herwig,
- Georgia Panopoulou,
- and Hans Lehrach
Genome Res. December 2003 13: 2736-2746; doi:10.1101/gr.1674103
...of the leading models for the analysis of the function of genomic regulatory networks that control development. We have analyzed a total of 107,283 cDNA clones of libraries that span the development of the sea urchin Strongylocentrotus purpuratus . Normalization by oligonucleotide fingerprinting, EST sequencing...
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RESOURCE: The Drosophila Gene Collection: Identification of Putative Full-Length cDNAs for 70% of D. melanogaster Genes
- Mark Stapleton,
- Guochun Liao,
- Peter Brokstein,
- Ling Hong,
- Piero Carninci,
- Toshiyuki Shiraki,
- Yoshihide Hayashizaki,
- Mark Champe,
- Joanne Pacleb,
- Ken Wan,
- Charles Yu,
- Joe Carlson,
- Reed George,
- Susan Celniker,
- and Gerald M. Rubin
Genome Res. August 1, 2002 12: 1294-1300; doi:10.1101/gr.269102
...of Drosophila melanogaster. Proc. Natl. Acad. Sci. 98 : 13225 – 13230 . ↵ Clark M.D. , Hennig S. , Herwig R. , Clifton S.W. , Marra M.A. , Lehrach H. , Johnson S.L. , GSC EST Group WU- ( 2001 ) An oligonucleotide fingerprint normalized and expressed sequence tag characterized zebrafish cDNA library. Genome Res...
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Method: Structure-optimized sgRNA selection with PlatinumCRISPr for efficient Cas9 generation of knockouts
- Irmgard U. Haussmann,
- Thomas C. Dix,
- David W.J. McQuarrie,
- Veronica Dezi,
- Abdullah I. Hans,
- Roland Arnold,
- and Matthias Soller
Genome Res. December 2024 34: 2279-2292; Published in Advance December 3, 2024, doi:10.1101/gr.279479.124
.... The presence of a PCR product indicates the expected gene deletion. DNA markers are indicated on the left. (C) Agarose gels showing RT-PCR products amplified from cDNA of control or Ythdc1 transposon excision lines using internal primers flanking an intron. Ectopic insertion in the opposite orientation...
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Review: Advancements in prospective single-cell lineage barcoding and their applications in research
- Xiaoli Zhang,
- Yirui Huang,
- Yajing Yang,
- Qi-En Wang,
- and Lang Li
Genome Res. December 2024 34: 2147-2162; Published in Advance November 21, 2024, doi:10.1101/gr.278944.124
...from one species to another (Le Douarin and Teillet 1973), injecting vital dyes into a single founder cell of transparent organisms like Caenorhabditis elegans and zebrafish (Lawson et al. 1986; Pedersen et al. 1986; Stern and Fraser 2001), or later through the introduction of reporter genes (Turner...
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RESOURCE: Fugu ESTs: New Resources for Transcription Analysis and Genome Annotation
- Melody S. Clark,
- Yvonne J.K. Edwards,
- Dan Peterson,
- Sandra W. Clifton,
- Amanda J. Thompson,
- Masahide Sasaki,
- Yutaka Suzuki,
- Kiyoshi Kikuchi,
- Shugo Watabe,
- Koichi Kawakami,
- Sumio Sugano,
- Greg Elgar,
- and Stephen L. Johnson
Genome Res. December 2003 13: 2747-2753; Published in Advance January 1, 2003, doi:10.1101/gr.1691503
...285 : 59 -68. ↵ Clark, M.D., Hennig, S., Herwig, R., Clifton, S.W., Marra, M.A., Lehrach, H., Johnson, S.L., and the WU-GSC EST Group. 2001 . An oligonucleotide fingerprint normalized and expressed sequence tag characterized zebrafish cDNA library. Genome Res. 11 : 1594 -1602. ↵ Claverie, J.M. 1997...
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PERSPECTIVE: Application of sequence-based methods in human microbial ecology
- Li Weng,
- Edward M. Rubin,
- and James Bristow
Genome Res. March 2006 16: 316-322; Published in Advance February 6, 2006, doi:10.1101/gr.3676406
...the use of DNA subtraction techniques to identify the Kaposi's sarcoma virus ( Chang et al. 1994 ), expression screening of a cDNA library with patient serum to identify the hepatitis-C agent ( Choo et al. 1989 ), and more recently hybridization-based screening to identify the SARS virus as a coronavirus...

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