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  1. Methods: Quantification of Multiple Gene Expression in Individual Cells
    • António Peixoto,
    • Marta Monteiro,
    • Benedita Rocha,
    • and Henrique Veiga-Fernandes
    Genome Res. October 2004 14: 1938-1947; doi:10.1101/gr.2890204
    ...randomness effects and demonstrating the high sensitivity of our amplification procedure. Finally, we tested the relative contribution of nonspecific signaling to our PCR read-outs. Indeed, SYBR Green incorporation in primers dimers could induce some type of background amplification. To evaluate...
  2. Method: Transcript amplification from single bacterium for transcriptome analysis
    • Yun Kang,
    • Michael H. Norris,
    • Jan Zarzycki-Siek,
    • William C. Nierman,
    • Stuart P. Donachie,
    • and Tung T. Hoang
    Genome Res. June 2011 21: 925-935; Published in Advance May 2, 2011, doi:10.1101/gr.116103.110
    ...containing a single bacterium was drawn and cut by the focused laser (green line) and catapulted at a distance from the cell with unfocused low-intensity laser beam (blue spot), which aseptically catapulted and isolated the single cell into the lid of a 0.2-mL PCR tube containing the cell lysis buffer. (D...
  3. Methods: Genome-wide detection and analysis of hippocampus core promoters using DeepCAGE
    • Eivind Valen,
    • Giovanni Pascarella,
    • Alistair Chalk,
    • Norihiro Maeda,
    • Miki Kojima,
    • Chika Kawazu,
    • Mitsuyoshi Murata,
    • Hiromi Nishiyori,
    • Dejan Lazarevic,
    • Dario Motti,
    • Troels Torben Marstrand,
    • Man-Hung Eric Tang,
    • Xiaobei Zhao,
    • Anders Krogh,
    • Ole Winther,
    • Takahiro Arakawa,
    • Jun Kawai,
    • Christine Wells,
    • Carsten Daub,
    • Matthias Harbers,
    • Yoshihide Hayashizaki,
    • Stefano Gustincich,
    • Albin Sandelin,
    • and Piero Carninci
    Genome Res. February 2009 19: 255-265; Published in Advance December 11, 2008, doi:10.1101/gr.084541.108
    ...′ end opened by MmeI digestion, CAGE tags were PCR amplified, purified, and further amplified before restriction and concatenation for direct sequencing (see Methods). The DeepCAGE technology does not require cloning in bacteria. View larger version: In this window In a new window Figure 1. Preparation...
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