Searching journal content for articles similar to Chen et al. 14 (4): 679.

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  1. ...of both the WGS and CBC methods, a “combined approach” for assembly was developed for the Rat Genome Sequencing Project (RGSP) ( Rat Genome Sequencing Project Consortium 2004 ). In the combined strategy, a BAC map is constructed and each BAC is sequenced to low (∼1×) coverage (“skimming”). WGS sequencing...
  2. ...) on mouse chromosome 5. Rat cDNA probes and specific sequence probes for all three GABAA receptor subunit genes have been used to initiate the construction of a sequence-ready contig of bacterial artificial chromosomes (BACs) encompassing this cluster. In the process of contig construction clones from 129...
  3. ...genetic disorders ( Iafrate et al. 2004 ; Sebat et al. 2004 ). Iafrate et al. (2004) used a bacterial artificial chromosome (BAC)-based array, with clones chosen at ∼1-megabase (Mb) intervals throughout the human , together with a technique called array-based comparative genomic hybridization (array CGH...
  4. ...to the repeat sequences. Two primary approaches can be taken to deal with complex s. The hierarchical shotgun approach involves generating an overlapping set of intermediate-sized clones [e.g., bacterial artificial chromosomes (BACs) with 200-kb inserts], selecting a tiling path of these clones...
  5. ...ignored mapping data or clone-based sequences during the assembly process ( Table 3 ). In contrast, build 29 was largely based on the hand-curated assembly of sequence overlaps from a BAC-based tiling path of clones (∼400 Mb of sequence) and represented a relatively purist form of hierarchical clone...
  6. ...program that could predict multiple and partial genes on both strands. This ability made Genscan suitable for annotating ORFs in anonymous DNA sequence such as that produced by sequencing random BAC clones. As the first GHMM suitable for annotating anonymous sequence, Genscan defined the state of the art...
  7. ...-ready map. This map comprises a minimal tiling path (MTP) of 95 clones from the C57BL/6 BAC library. Sequence assembly of the finished sequence generated from the MTP produced a single contig of 12,660,359 bp that extends across chromosome bands A3.1-A4, encompassing both light and dark staining Giemsa...
  8. ...clones from the Washington University fpc fingerprints ( Wallis et al. 2004 ). Sequencing and assembly BACs selected to create an efficient tiling path were isolated, fragmented, subcloned, and sequenced in both directions as previously described ( Dehal et al. 2001 , Detter et al. 2002 ). Detailed...
  9. .....org means of a two-step approach based on intraspecific aCGH. In a first phase (discovery phase), we used a -wide tiling-path 32K BAC array to discover CNV regions in chimpanzees, gorillas, and orangutans. In a posterior Refinement Phase we validated these CNVs, refined their boundaries, and discovered new...
  10. .... Summarized log2 ratios were calculated by taking a mean across duplicate arrays. Dye-swap hybridization for each pair of arrays was performed to normalize for any dye bias. The genomic position of the BACs used to generate the MHC tile path arrays was established using the NCBI build 34 assembly of the human...
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