Searching journal content for articles similar to Chen et al. 14 (10b): 2155.

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  1. ...the standard ONT protocol was use of SuperScript IV (Thermo Fisher Scientific) for reverse transcription. RNA sequencing on the GridION platform was performed using ONT R9.4 flow cells and the standard MinKNOW protocol script (NC_48Hr_sequencing_FLO-MIN106_SQK-RNA001).Preprocessing and alignmentsReads were...
  2. ...transcription and massively parallel sequencing (Helm and Motorin 2017; Yang et al. 2018). However, it provides poor resolution in terms of modification site. Third generation sequencing technology, for example, the Pacific Biosciences (PacBio) RSII platform, is able to produce long reads and detect DNA...
  3. ...previously annotated exons. Our results show that as much as 20% of the C. elegans genome may be incorrectly annotated. Many annotation errors could be corrected proactively with our large-scale RACE platform. Footnotes ↵ 5 Corresponding authors. E-mail kourosh...
  4. .... 268 : 78 -94. ↵ C. elegans Sequencing Consortium. 1998 . Genome sequence of the nematode C. elegans : A platform for investigating biology. Science 282 : 2012 -2018. ↵ Cho, S., Suk-Won, J., Cohen, A., and Ellis, R. 2004 . A phylogeny of Caenorhabditis reveals frequent loss of introns during nematode...
  5. .... Genome sequence of the nematode C. elegans : A platform for investigating biology. Science 282 : 2012 -2018. ↵ Cliften, P.F., Hillier, L.W., Fulton, L., Graves, T., Miner, T., Gish, W.R., Waterston, R.H., and Johnston, M. 2001 . Surveying Saccharomyces s to identify functional elements by comparative DNA...
  6. .... elegans ORFeome resource contains 11,942 cloned ORFs ( Reboul et al. 2003 ) and constitutes a platform for proteomic and functional genomic approaches ( Reboul et al. 2003 ; Rual et al. 2004 ). The C. elegans ORFeome resource is dynamic, with new ORFs added or corrected continuously ( Lamesch et al. 2004...
  7. ...from sets 1-3 that we had not previously assayed by injection ( Piano et al. 2002 ) for characterization in this study (see Fig. 3 ). After completing the RNAi analysis, we remapped this set of ORFeome clones and the PCR products used to define the ovary-enriched gene set to the latest WormBase freeze...
  8. ...layer of differential miRNA expression in nematodes. The data and transgenic lines that we present provide a platform for functional miRNA studies to delineate their roles in the development of the animal and to understand their function in gene regulatory networks. Results Generation of transgenic Pmi...
  9. ..., S., et al. 2002 . Structural genomics: A pipeline for providing structures for the biologist. Protein Sci. 11 : 723 -738. ↵ Chen, N., Lawson, D., Bradnam, K., Harris, T.W., and Stein, L.D. 2004 . WormBase as an integrated platform for the C. elegans ORFeome. Genome Res. (this issue). ↵ Cheo, D...
  10. ...is a substantially altered view of the C. elegans protein-coding potential that will be important to integrate into WormBase annotations ( Table 6 ). View this table: In this window In a new window Table 6. Summary of features identified Development of rigorous statistical approaches to these short, error...
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