Searching journal content for articles similar to Chaisson et al. 19 (2): 336.

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  1. ...). In this work we focus on the evaluation of methods for de novo assembly using low-cost ‘‘short read’’ technology, where the reads are comparatively short in length but large in number, the sequences being assembled represent a novel diploid and the nearest homologous to that being assembled is significantly...
  2. ...to Sanger-based sequencing. Current fragment assembly algorithms have been implemented and optimized for mate-paired Sanger-based reads, and thus do not perform well on short reads produced by short read technologies. We present a new Eulerian assembler that generates nearly optimal short read assemblies...
  3. ...ECHO: A reference-free short-read error correction algorithm Wei-Chun Kao 1 , Andrew H. Chan 1 and Yun S. Song 1 , 2 , 3 1Computer Science Division, University of California, Berkeley, California 94721, USA; 2Department...
  4. ...-end and mate-pair sequencing to similar amounts of coverage. To investigate the relevant performance of long and short reads for cancer analysis, we perform an array of comparisons in parallel using both technologies.Read mapping and copy number analysisLong reads have more information to uniquely align...
  5. ..., that of the dog, to which it is ∼80% identical at the nucleotide level, and Pontius et al. (2007 , this issue) make effective use of it. In its most extreme version, assisted assembly consists simply of aligning reads to the reference , using read mate-pair information to reduce the risk of misassembly due...
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  6. ...correction methods can be very effective when coverage is deep, as it often is with newer short-read sequencing projects. The scaffolding phase of assembly focuses on resolving repeats by linking the initial contigs into scaffolds, guided by mate-pair data. Mate pairs constrain the separation distance...
  7. ...–1623. Cardle L, Ramsay L, Milbourne D, Macaulay M, Marshall D, Waugh R. 2000. Computational and experimental characterization of physically clustered simple sequence repeats in plants. Genetics 156: 847–854. Chaisson MJ, Brinza D, Pevzner PA. 2009. De novo fragment assembly with short mate-paired reads: Does...
  8. ...fragment assembly with short mate-paired reads: Does the read length matter? Genome Res 19: 336–346. Chen K,Wallis J, McLellanM, Larson D, Kalicki J, Pohl C,McGrath S,Wendl M, Zhang Q, Locke D, et al. 2009. BreakDancer: An algorithm for highresolutionmapping of genomic structural variation.NatMethods 6...
  9. ...of short fragments, suggesting an opportunity to assemble SVs into contigs. Using SV-derived reads, 1101 were de novo assembled with a mean length of 579 bp and a longest contig of 2826 bp from LIG3−/−:NC3: sample 53 (Supplemental Methods). We performed Sanger sequencing of four contigs to validate...
  10. ...cell using the previously synthesized strand as template. Likewise, the ABI approach to obtaining mate-pair sequence appears to be priming a ligation series from the 5′ end of the first and then second stand of a template. The superior read lengths offered by the 454 system are a distinct advantage...
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