Searching journal content for articles similar to Carninci et al. 13 (6b): 1273.

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  1. ...). These results suggested that tumor cells with IPA of ERCC1 would have dysregulated DNA repair functions, which might contribute to HCC development.Finally, we validated these two candidates by performing a 3′ rapid amplification of cDNA ends (3′ RACE) experiment in the liver cancer cell line HepG2. As shown...
  2. ...-cell RNA-seq methods also rely on the end sequencing of cDNA molecules and thus largely fail to capture alternative splicing events.To address the need for a more comprehensive mRNA data set that captures the transcripts in specific tissues during embryonic development, we have used fluorescence...
  3. ..., because the mammalian transcriptome has been incomplete. The RIKEN Mouse Encyclopedia project aims to make a library of all transcribed sequences as cDNA clones ( The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium 2001 ). Analysis of the expression pattern for these cDNAs...
  4. ...; ‘t Hoen et al. 2008; Suzuki et al. 2009; Valen et al. 2009; Levin et al. 2010; Plessy et al. 2010; Wu et al. 2010; Sam et al. 2011). The majority of transcriptome protocols running on second-generation sequencing platforms have relied on two PCR amplification steps: one for preamplication of cDNA...
  5. ...various combinations of alternative splicing, transcription initiation, and polyadenylation. The enormous complexity of the mammalian transcriptome was first exposed by large-scale sequencing of full-length cDNA transcripts frommouse (Okazaki et al. 2002;Maeda et al. 2006) and chromosome tiling array...
  6. ...). Accordingly, the average efficiencies of each target arewithin 4.1%differences (<5%) andpermit accurate analysis. The expression level of mRNA BTH_I2028 was taken as 1 for each amplified sample tested, and the amounts of tRNA and rRNAs were normalized relative to this value. cDNA library construction...
  7. ...encyclopedia. Genome Res . (this issue). ↵ The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team. 2002 . Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature 420 : 563 -573. ↵ The Gene Ontology Consortium. 2001 . Creating...
  8. ...disease loci. These data provide a major resource for understanding the role of imprinting in mammalian inherited traits. The FANTOM2 set of mouse full-length cDNAs has provided the first global view of a mammalian transcriptome and is an essential resource for annotation ( The FANTOM Consortium...
  9. ...Enzymol. 303 : 19 -44. ↵ Carninci, P., Waki, K., Shiraki, T., Konno, H., Shibata, K., Itoh, M., Aizawa, K., Arakawa, T., Ishii, Y., Sasaki, D., et al. 2003 . Targeting a complex transcriptome: The construction of the mouse full-length cDNA encyclopedia. Genome Res. (this issue). ↵ Catterall, W.A. 2000...
  10. ...an incomplete and imperfect prediction and description of the full-length transcripts and splice variants that can be transcribed from the . The RIKEN Mouse Gene Encyclopedia Project has provided the most comprehensive collection of full-length mammalian complementary DNAs (cDNAs; Okazaki et al. 2002...
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