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  1. ...sequencing of individual BACs. Short-tag pooled genomic indexing (ST-PGI) further improves the economy of mapping by sequencing multiple, short, mapable tags within a single sequencing reaction. Shotgun sequencing of pooled BACs has been previously proposed in the context of the clone-array pooled shotgun...
  2. ...resistance gene Gpa2 in potato. Theor Appl Genet 106: 1524–1531. Borm TJA. 2008. ‘‘Construction and use of a physical map of potato.’’ PhD thesis, Wageningen University, The Netherlands. CaiW-W,ChenR, Gibbs RA, Bradley A. 2001. AClone-Array Pooled Shotgun Strategy for sequencing large s. Genome Res 11: 1619...
  3. ...for shotgun sequencing strategies and have become the main sequence-ready clone resources for use in large-scale mapping and sequencing efforts ( Venter et al. 1996 ; Gregory et al. 1997 ; Marra et al. 1997 ). 129S6/SvEvTac and C57BL/6J female mice ( Simpson et al. 1997 ) were selected as the most useful...
  4. ..., R.A., and Bradley, A. 2001 . A clone-array pooled shotgun strategy for sequencing large s. Genome Res. 11 : 1619 -1623. ↵ Engler, F.W., Hatfield, J., Nelson, W., and Soderlund, C.A. 2003 . Locating sequence on FPC maps and selecting a minimal tiling path. Genome Res. 13 : 2152 -2163. ↵ Gordon, D...
  5. ...shotgun strategy for sequencing large s. Genome Res 11: 1619– 1623. CsurosM, Li B, Milosavljevic A. 2003. Clone-array pooled shotgunmapping and sequencing: Design and analysis of experiments.Genome Inform 14: 186–195. FreemanWM, Walker SJ, Vrana KE. 1999. Quantitative RT-PCR: Pitfalls and potential...
  6. ...and minimally overlapping BAC clones for sequencing. BAC clones were full-shotgun sequenced at the National Institutes of Health (NIH) Intramural Sequencing Center and submitted to GenBank immediately after assembly. The sequence from each clone was then evaluated to determine whether or not it was orthologous...
  7. ...an equivalent probe in the population derived from the whole- shotgun sequence reads. These results indicate that the described approach for universal probe design and multispecies BAC mapping should be applicable to virtually the entire human using human and mouse genomic sequence data that now are readily...
  8. ...of genomic libraries mostly as a result of their smaller vector size (about 8 kb versus about 15 kb) because of the resulting favorable effect on the economy of clone-by-clone shotgun sequencing. A further reason for the use of the BAC libraries was that the earlier rat PAC library (RPCI-31) was prepared...
  9. ...(Supplemental data). However, we feel that the sample size is not large enough to make any definitive predictions. This genomic segment is well represented in the whole- shotgun (wgs) trace files, as well as trace files obtained by the Clone-Array Pooled Shotgun Sequencing (CAPPS; Cai et al. 2001 ) method...
  10. ...shotgun DNA sequence data. It allows the use of entire reads independent of data quality, uses a combination of user-supplied and internally computed data quality information to improve the accuracy of assembly in the presence of repeats, and constructs contig sequence as a mosaic of the highest quality...
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