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  1. ...-Lloyd, B.V. and Newbury. H.J. 1992 . The random amplification of polymorphic DNA for fingerprinting plants. PCR Methods Applic. 1 : 274 – 276 . 64. Blanchard, M.M., Taillon-Miller, P. Nowotny, P. and Nowotny. V. 1993 . PCR buffer optimization with uniform temperature regimen to facilitate automation. PCR...
  2. ..., hinging on the precise binding of transcription factors (TFs) and cofactors to gene regulatory elements such as promoters and enhancers. Although it is relatively routine to profile -wide DNA binding landscapes of proteins, identifying the specific proteins that bind to, and regulate the transcription of...
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  3. ...min. After a 15-rain centrifugation (16,000g), the DNA pel- let was washed once with 70% ethanol, dried in vacuo, and resuspended in 50 B1 of TE buffer (10 mM Tris-HC1, pH 8.0, 1 mM EDTA). Special attention should be paid to the DNA pellet be- cause pellet loss after precipitation is the main reason...
  4. ...,000oligonucleotide probes, 25 nucleotides in length, were designed to screen for all possible heterozygous germ-line mutations in the 9.17-kb coding region of the ATM gene. A strategy for rapidly developing multiexon PCR amplification protocols in DNA chip-based hybridization analysis was devised and implemented...
  5. ...., , Parik, J. , Malmgren, H. , Stewart, J. , Pettersson, U. , Landegren. U. Lagerstrom, M., Parik, J. Malmgren, H. Stewart, J. Pettersson, U. and Landegren. U. 1991 . Capture PCR: Efficient amplification of DNA fragments adjacent to a known sequence in human and YAC DNA. PCR Methods Applic. 1 : 111 – 119...
  6. ...sequence tags (ISTs) Genomic DNA was isolated in 96-well format from confluent G418-resistant ES cell clones. Cells were rinsed twice with PBS and frozen at −80°C prior to processing. ES cell clone samples were treated with 50 μL of lysis buffer (50 mM Tris at pH 7.5, 50 mM EDTA at pH 8.0, 100 mM NaCl, 1...
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