Searching journal content for articles similar to Cadwell and Joyce 2 (1): 28.

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  1. Method: High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism, Bombyx mori
    • Sanyuan Ma,
    • Tong Zhang,
    • Ruolin Wang,
    • Pan Wang,
    • Yue Liu,
    • Jiasong Chang,
    • Aoming Wang,
    • Xinhui Lan,
    • Le Sun,
    • Hao Sun,
    • Run Shi,
    • Wei Lu,
    • Dan Liu,
    • Na Zhang,
    • Wenbo Hu,
    • Xiaogang Wang,
    • Weiqing Xing,
    • Ling Jia,
    • and Qingyou Xia
    Genome Res. January 2024 34: 134-144; Published in Advance January 8, 2024, doi:10.1101/gr.278297.123
    ...libraries, such as those generated through radiation mutagenesis, chemical mutagenesis, random transgenic insertion, or transgenic RNAi (Venken et al. 2014).We established a -wide CRISPR screen platform in cultured B. mori cells and showed its simplicity and efficiency in identifying genes essential...
  2. Resource: Genome-scale targeted mutagenesis in Brassica napus using a pooled CRISPR library
    • Jianjie He,
    • Kai Zhang,
    • Shuxiang Yan,
    • Mi Tang,
    • Weixian Zhou,
    • Yongtai Yin,
    • Kang Chen,
    • Chunyu Zhang,
    • and Maoteng Li
    Genome Res. May 2023 33: 798-809; Published in Advance June 8, 2023, doi:10.1101/gr.277650.123
    ...studies. Our research provides valuable resources for functional genomics, elite crop breeding, and a good reference for high-throughput targeted mutagenesis in other polyploid plants.Routine strategies for random mutagenesis, including physical (Henry et al. 2015; Li et al. 2017), chemical (Tang et al...
  3. Research: Saturation mutagenesis reveals manifold determinants of exon definition
    • Shengdong Ke,
    • Vincent Anquetil,
    • Jorge Rojas Zamalloa,
    • Alisha Maity,
    • Anthony Yang,
    • Mauricio A. Arias,
    • Sergey Kalachikov,
    • James J. Russo,
    • Jingyue Ju,
    • and Lawrence A. Chasin
    Genome Res. January 2018 28: 11-24; Published in Advance December 14, 2017, doi:10.1101/gr.219683.116
    ...). This approach has now been extended to mutagenesis of endogenous (Findlay et al. 2014) as well as exogenous (Julien et al. 2016) exons and to surveys of long random sequences (Rosenberg et al. 2015). In the work described here, we have used deep sequencing to examine the splicing phenotypes produced...
  4. Research: Nascent RNA folding mitigates transcription-associated mutagenesis
    • Xiaoshu Chen,
    • Jian-Rong Yang,
    • and Jianzhi Zhang
    Genome Res. January 2016 26: 50-59; Published in Advance October 30, 2015, doi:10.1101/gr.195164.115
    ...Nascent RNA folding mitigates transcription-associated mutagenesis Xiaoshu Chen , Jian-Rong Yang and Jianzhi Zhang Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan 48109, USA Corresponding...
  5. Resource: Large-scale discovery of mouse transgenic integration sites reveals frequent structural variation and insertional mutagenesis
    • Leslie O. Goodwin,
    • Erik Splinter,
    • Tiffany L. Davis,
    • Rachel Urban,
    • Hao He,
    • Robert E. Braun,
    • Elissa J. Chesler,
    • Vivek Kumar,
    • Max van Min,
    • Juliet Ndukum,
    • Vivek M. Philip,
    • Laura G. Reinholdt,
    • Karen Svenson,
    • Jacqueline K. White,
    • Michael Sasner,
    • Cathleen Lutz,
    • and Stephen A. Murray
    Genome Res. March 2019 29: 494-505; Published in Advance January 18, 2019, doi:10.1101/gr.233866.117
    ..., providing numerous models of human disease and critical genetic tools in widespread use today. Generated through the random integration of DNA fragments into the host , transgenesis can lead to insertional mutagenesis if a coding gene or an essential element is disrupted, and there is evidence that larger...
    OPEN ACCESS ARTICLE
  6. Research: Transposon insertional mutagenesis in Saccharomyces uvarum reveals trans-acting effects influencing species-dependent essential genes
    • Monica R. Sanchez,
    • Celia Payen,
    • Frances Cheong,
    • Blake T. Hovde,
    • Sarah Bissonnette,
    • Adam P. Arkin,
    • Jeffrey M. Skerker,
    • Rachel B. Brem,
    • Amy A. Caudy,
    • and Maitreya J. Dunham
    Genome Res. March 2019 29: 396-406; Published in Advance January 11, 2019, doi:10.1101/gr.232330.117
    ...-based mutagenesis methods used to create random insertional mutant collections, eliminating requirements for a priori knowledge about defined coding regions and providing information about partial loss-of-function or gain-of-function mutations. Random insertional profiling has been widely applied across various...
  7. Method: Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium
    • Cheng-Lin Frank Li,
    • Balaji Santhanam,
    • Amanda Nicole Webb,
    • Blaž Zupan,
    • and Gad Shaulsky
    Genome Res. September 2016 26: 1268-1276; Published in Advance June 15, 2016, doi:10.1101/gr.205682.116
    ...mutagenesis produces dominant alleles as well. Some analyses of chemical mutagenesis data were based on the assumption that mutations occur at random (Loomis 1987). These analyses were remarkably accurate despite our finding that NTG mutagenesis is biased toward exons of coding genes. This bias is a combined...
  8. Method: A versatile type VI CRISPR-based approach for targeted m6A demethylation in mRNAs
    • Panagiotis G. Adamopoulos,
    • Konstantina Athanasopoulou,
    • and Andreas Scorilas
    Genome Res. January 2026 36: 169-182; Published in Advance December 11, 2025, doi:10.1101/gr.280476.125
    ...-Vec expressing a catalytically inactive ALKBH5 (dALKBH5) after site-directed mutagenesis leading to the H204A mutant enzyme. (A) Sanger sequencing results validating the successful generation of Dem6A-Vec expressing dALKBH5. (B) SELECT-qPCR results for m6A:2886 of EEF2 and m6A:418 of RRAGA on WT HeLa cells...
  9. Method: Restoring the potency of neutralizing antibody via guided hypermutation with hyper-antibody editor
    • Yuhong Wang,
    • Yuefeng Guo,
    • Qiuyu Lu,
    • Xinyi Liu,
    • Haoqi Xu,
    • Jiayi Chen,
    • Ruiqi Pi,
    • Shaopeng Yuan,
    • Ziting Yang,
    • Rusen Lu,
    • Fei-Long Meng,
    • Tingting Gan,
    • and Jiazhi Hu
    Genome Res. March 19, 2026 gr.281396.125; Published in Advance March 19, 2026, doi:10.1101/gr.281396.125
    ...approaches require the development of specialized mouse models. Alternatively, classical directed 53 evolution and continuous evolution methods have been applied to optimize antibody affinity in vitro 54 or in cultured cells (Molina et al. 2022). Classical directed evolution relies on random mutagenesis 55...
    ACCEPTED MANUSCRIPT
  10. Methods: Efficient Target-Selected Mutagenesis in Zebrafish
    • Erno Wienholds,
    • Freek van Eeden,
    • Marit Kosters,
    • Josine Mudde,
    • Ronald H.A. Plasterk,
    • and Edwin Cuppen
    Genome Res. December 2003 13: 2700-2707; Published in Advance January 1, 2003, doi:10.1101/gr.1725103
    ...by nuclear transfer of genetically modified cultured cells ( Lee et al. 2002 ). However, this has also not resulted in a targeted knockout. Recently, we have shown that reverse genetic analysis of gene function in zebrafish is possible by target-selected mutagenesis ( Wienholds et al. 2002 ); random...
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