Searching journal content for articles similar to Borén et al. 6 (11): 1123.

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  1. GENOME METHODS: Modification of Bacterial Artificial Chromosome Clones Using Cre Recombinase: Introduction of Selectable Markers for Expression in Eukaryotic Cells
    • Su Young Kim,
    • Stephen K. Horrigan,
    • Janine L. Altenhofen,
    • Zarema H. Arbieva,
    • Ronald Hoffman,
    • and Carol A. Westbrook
    Genome Res. April 1, 1998 8: 404-412; doi:10.1101/gr.8.4.404
    ...A-assisted restriction endonuclease cleavage of large insert clones allows for site-specific mutagenesis and also deletions and fusions of BAC clones ( Boren et al. 1996 ). These advances, in combination with BAC retrofitting, will permit researchers to examine the biological effects of wild-type genomic DNA contained...
  2. METHODS: Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using Yeast
    • Christopher K. Raymond,
    • Elizabeth H. Sims,
    • and Maynard V. Olson
    Genome Res. January 1, 2002 12: 190-197; doi:10.1101/gr.205201
    ...– 246 . ↵ Hill F. , Benes V. , Thomasova D. , Stewart A. , Kafatos F. , Ansorge W. ( 2000 ) BAC trimming: minimizing clone overlaps. Genomics 64 : 111 – 113 . ↵ Hoffman C. , Winston F. ( 1987 ) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation...
  3. Method: A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor
    • Hengxing Lu,
    • Jun Liu,
    • Tao Feng,
    • Zihang Guo,
    • Yunjun Yin,
    • Fei Gao,
    • Gengsheng Cao,
    • Xuguang Du,
    • and Sen Wu
    Genome Res. May 2021 31: 900-909; Published in Advance April 1, 2021, doi:10.1101/gr.271312.120
    ...strategy, homology-independent targeted trapping (HIT-trapping), which is generic and can efficiently target-trap an endogenous gene of interest independent of homology arm and embryonic stem cells. Further optimization and equipping the HIT-trap donor with a site-specific DNA inversion mechanism enabled...
  4. Method: 2C-Cas9: a versatile tool for clonal analysis of gene function
    • Vincenzo Di Donato,
    • Flavia De Santis,
    • Thomas O. Auer,
    • Noé Testa,
    • Héctor Sánchez-Iranzo,
    • Nadia Mercader,
    • Jean-Paul Concordet,
    • and Filippo Del Bene
    Genome Res. May 2016 26: 681-692; Published in Advance March 8, 2016, doi:10.1101/gr.196170.115
    ...in retinal progenitor cells. (A) (Upper panel) Differentiated RGCs are detected in wild-type clones injected with the DNA construct containing control sgRNAs. (Lower panel) GFP-labeled mutant clones show reduction of RGCs in the retinal section of a 5 dpf larva, as expected from atoh7 loss...
  5. LETTER: An infectious progenitor for the murine IAP retrotransposon: Emergence of an intracellular genetic parasite from an ancient retrovirus
    • David Ribet,
    • Francis Harper,
    • Anne Dupressoir,
    • Marie Dewannieux,
    • Gérard Pierron,
    • and Thierry Heidmann
    Genome Res. April 2008 18: 597-609; Published in Advance February 6, 2008, doi:10.1101/gr.073486.107
    ...4.6- and 4.2-kb elements ( Fig. 1C ). It is likely that these full-length and deleted elements correspond to the IAPE-A, -B, and -C elements, respectively, previously identified by Southern Blot analysis of EcoRI digests of mouse genomic DNA ( Reuss et al. 1996 ). Of note, most IAPE-A copies...
  6. Method: A mobile insulator system to detect and disrupt cis-regulatory landscapes in vertebrates
    • José Bessa,
    • Mario Luengo,
    • Solangel Rivero-Gil,
    • Ana Ariza-Cosano,
    • António H.F. Maia,
    • Francisco J. Ruiz-Ruano,
    • Pablo Caballero,
    • Silvia Naranjo,
    • Jaime J. Carvajal,
    • and José Luis Gómez-Skarmeta
    Genome Res. March 2014 24: 487-495; Published in Advance November 25, 2013, doi:10.1101/gr.165654.113
    ...(primer sequences are available in Supplemental Table 3) using a mix of 80% epiboly to 48-hpf retro-transcribed cDNA as template (SuperScript II, Invitrogen). After PCR amplification, specific cDNAs were cloned in a pGEM-T easy vector (Promega). These clones were linearized with restriction enzymes...
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