Searching journal content for articles similar to Bono et al. 13 (6b): 1318.

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  1. ...). Notably, MAPCap works well with as little as 100 ng total RNA input.A number of other TSS profiling methods use an oligo capping approach, which involves enzymatic removal of the m7G cap and replacement with a synthetic oligo, allowing selection of 5′-complete cDNAs (Suzuki and Sugano 2003; Wakaguri et al...
  2. ...transcriptase adds nontemplated nucleotides to cDNAs during 59-RACE and primer extension. Biotechniques 30: 574–580, 582. Cloonan N, Forrest AR, Kolle G, Gardiner BB, Faulkner GJ, Brown MK, Taylor DF, Steptoe AL, Wani S, Bethel G, et al. 2008. Stem cell transcriptome profiling via massive-scale mRNA sequencing...
  3. ...-950. ↵ Bono, H., Yagi, K., Kasukawa, T., Nikaido, I., Tominaga, N., Miki, R., Mizuno, Y., Tomaru, Y., Goto, H., Nitanda, H., et al. 2003 . Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarrays. Genome Res. (this issue). ↵ Bortolin, M.L. and Kiss. T. 1998 . Human U19...
  4. ...junction spanning reads in those RNA-seq profiles revealed a much richer set of processed transcripts. Additional RNA-seq data sets, along with cap analysis of gene expression (CAGE-seq) and full-insert cDNA sequencing, have been used to generate the modENCODE D. melanogaster transcriptome annotation...
  5. ...transcript repertoire is much more diverse than anticipated. In part, transcript diversity is generated through the use of alternative promoters and alternate splicing. In addition, based on discoveries using technologies such as full-length cDNA libraries and whole genome tiling microarrays, it is now...
  6. ..., Didier Samuel 2 , 3 , Gioacchino Natoli 4 , Marie Annick Buendia 2 , 3 , Jamila Faivre 2 , 3 , 7 and Piero Carninci 1 1 RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Kanagawa, 230-0045 Japan...
  7. ...) ( Kamiya et al. 2000 ). To facilitate positional candidate cloning, it is also useful to know the tissue-expression profile of each gene. With this in mind, we have attempted to draw a picture of tissue expression profiles in 49 tissues using the RIKEN 19K cDNA microarray ( Miki et al. 2001 ). Further...
  8. ...of the mouse transcriptome using RIKEN cDNA microarrays. Genome Res . (this issue). ↵ Carninci, P., Waki, K., Shiraki, T., Konno, H., Shibata, K., Itoh, M., Aizawa, K., Arakawa, T., Ishii, Y., Sasaki, D., et al. 2003 . Targeting a complex transcriptome: The construction of the mouse full-length cDNA...
  9. ...Inferring Alternative Splicing Patterns in Mouse from a Full-Length cDNA Library and Microarray Data Hiromi Kochiwa 1 , 3 , Ryosuke Suzuki 1 , 3 , Takanori Washio 3 , Rintaro Saito 4 , The RIKEN Genome Exploration Research Group Phase, II Team...
  10. ...in mouse, or that there is a separate enzyme yet to be identified. A line of evidence that supports the former hypothesis was obtained from the microarray data. We examined the expression profile of the genes on this pathway using the data of the RIKEN 20k mouse cDNA microarray (Nos. 1–3, a total of ∼ 60K...
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