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Bochner et al. 11 (7): 1246
.
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19
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METHODS
:
High-Throughput Variation Detection and Genotyping Using Microarrays
David J. Cutler
,
Michael E. Zwick
,
Minerva M. Carrasquillo
,
Christopher T. Yohn
,
Katherine P. Tobin
,
Carl Kashuk
,
Debra J. Mathews
,
Nila A. Shah
,
Evan E. Eichler
,
Janet A. Warrington
,
and
Aravinda Chakravarti
Genome Res.
November 1, 2001
11
:
1913
-
1925
;
Published in Advance
October 15, 2001
,
doi:
10.1101/gr.197201
...
High-Throughput
Variation Detection and Genotyping Using Microarrays David J. Cutler 1 , 4 , Michael E. Zwick 1 , Minerva M. Carrasquillo 3 , Christopher T. Yohn 1 , Katherine P. Tobin 1 , Carl Kashuk 1 , Debra J. Mathews 3...
Abstract
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Methods
:
High-Throughput Selection of Effective RNAi Probes for Gene Silencing
Rajeev Kumar
,
Douglas S. Conklin
,
and
Vivek Mittal
Genome Res.
October 2003
13
:
2333
-
2340
;
doi:
10.1101/gr.1575003
...effective siRNA against any
gene
. The efficacy of siRNA sequences is monitored by their ability to reduce the expression of cognate target-reporter fusions with easily quantified readouts. Finally, using
micro
array
-based cell transfections, we demonstrate an unlimited potential of this approach in
high-throughput
...
Abstract
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Method
:
Gene expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ
Joseph W. Foley
,
Chunfang Zhu
,
Philippe Jolivet
,
Shirley X. Zhu
,
Peipei Lu
,
Michael J. Meaney
,
and
Robert B. West
Genome Res.
November 2019
29
:
1816
-
1825
;
Published in Advance
September 13, 2019
,
doi:
10.1101/gr.234807.118
...macrophages and single ductal cells inferred to be macrophages (Supplemental File 3). Many of the most significant
genes
are related to extracellular matrix interactions and leukocyte signaling. These data raise the possibility that intraductal macrophages have a
phenotype
that is distinct from stromal...
Abstract
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Research
:
Genome-scale RNAi on living-cell microarrays identifies novel regulators of
Drosophila melanogaster
TORC1–S6K pathway signaling
Robert A. Lindquist
,
Kathleen A. Ottina
,
Douglas B. Wheeler
,
Peggy P. Hsu
,
Carson C. Thoreen
,
David A. Guertin
,
Siraj M. Ali
,
Shomit Sengupta
,
Yoav D. Shaul
,
Michael R. Lamprecht
,
Katherine L. Madden
,
Adam R. Papallo
,
Thouis R. Jones
,
David M. Sabatini
,
and
Anne E. Carpenter
Genome Res.
March 2011
21
:
433
-
446
;
Published in Advance
January 14, 2011
,
doi:
10.1101/gr.111492.110
...
genes
as significant on-target regulators of RPS6 phosphorylation, and we characterized them with extensive secondary
assays
probing various arms of the TORC1 pathways, identifying functional relationships among those
genes
. We conclude that cell-based microarrays are a useful platform for genome...
Abstract
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METHODS
:
Rapid Mapping of Zebrafish Mutations With SNPs and Oligonucleotide Microarrays
Heather L. Stickney
,
Jeremy Schmutz
,
Ian G. Woods
,
Caleb C. Holtzer
,
Mark C. Dickson
,
Peter D. Kelly
,
Richard M. Myers
,
and
William S. Talbot
Genome Res.
December 1, 2002
12
:
1929
-
1934
;
doi:
10.1101/gr.777302
...) Oligonucleotide arrays for
high-throughput
SNPs detection in the MHC class I
genes
: HLA-B as a model system. Genome Res. 12 : 447 – 457 . ↵ Hacia J.G. , Fan J.B. , Ryder O. , Jin L. , Edgemon K. , Ghandour G. , Mayer R.A. , Sun B. , Hsie L. , Robbins C.M. , et al. ( 1999 ) Determination of ancestral alleles...
Abstract
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METHODS
:
High-Density Cell Microarrays for Parallel Functional Determinations
C. Wilson Xu
Genome Res.
March 1, 2002
12
:
482
-
486
;
Published in Advance
February 15, 2002
,
doi:
10.1101/gr.213002
...deletion strains could be arrayed on the permeable membrane within an area of ∼6 cm2. Such cell microarrays would allow
high-throughput
phenotypic
assays
(e.g., cell shape, budding pattern, and drug resistance) of
gene
activities under a variety of conditions. Therefore, cell microarrays should complement...
Abstract
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REVIEW
:
Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends
Deirdre Meldrum
Genome Res.
September 1, 2000
10
:
1288
-
1303
;
doi:
10.1101/gr.157400
.... The microarray platform is in a 96-well customizable format for medium-density and
high-throughput
and up to 250
genes
/array ( Lamture et al. 1994 ). In September 1999, Genometrix and Motorola, Inc. agreed to a coexclusive rights agreement to use and commercialize proprietary Genometix electronic DNA chip...
Abstract
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METHODS
:
Y-Chromosomal SNPs in Finno–Ugric-Speaking Populations Analyzed by Minisequencing on Microarrays
Mirja Raitio
,
Katarina Lindroos
,
Minna Laukkanen
,
Tomi Pastinen
,
Pertti Sistonen
,
Antti Sajantila
,
and
Ann-Christine Syvänen
Genome Res.
March 1, 2001
11
:
471
-
482
;
doi:
10.1101/gr.156301
...-based multiplex analysis of candidate
genes
reveals two independent and additive genetic risk factors for myocardial infarction in the Finnish population. Hum. Mol. Genet. 7 : 1453 – 1462 . ↵ Pastinen T. , Raitio M. , Lindroos K. , Tainola P. , Peltonen L. , Syvänen A.-C. ( 2000 ) A system for specific,
high-throughput
...
Abstract
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Resource
:
A spatial and temporal map of
C. elegans
gene expression
W. Clay Spencer
,
Georg Zeller
,
Joseph D. Watson
,
Stefan R. Henz
,
Kathie L. Watkins
,
Rebecca D. McWhirter
,
Sarah Petersen
,
Vipin T. Sreedharan
,
Christian Widmer
,
Jeanyoung Jo
,
Valerie Reinke
,
Lisa Petrella
,
Susan Strome
,
Stephen E. Von Stetina
,
Menachem Katz
,
Shai Shaham
,
Gunnar Rätsch
,
and
David M. Miller III
Genome Res.
February 2011
21
:
325
-
341
;
Published in Advance
December 22, 2010
,
doi:
10.1101/gr.114595.110
...A spatial and temporal map of C. elegans
gene
expression W. Clay Spencer 1 , 7 , Georg Zeller 2 , 3 , 7 , 8 , Joseph D. Watson 1 , 9 , Stefan R. Henz 3 , Kathie L. Watkins 1 , Rebecca D. McWhirter 1 , Sarah Petersen...
Abstract
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Methods
:
Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites
George A. Follows
,
Pawan Dhami
,
Berthold Göttgens
,
Alexander W. Bruce
,
Peter J. Campbell
,
Shane C. Dillon
,
Aileen M. Smith
,
Christoph Koch
,
Ian J. Donaldson
,
Mike A. Scott
,
Ian Dunham
,
Mary E. Janes
,
David Vetrie
,
and
Anthony R. Green
Genome Res.
October 2006
16
:
1310
-
1319
;
Published in Advance
September 8, 2006
,
doi:
10.1101/gr.5373606
...is available from the authors on request. Transfection experiments PCR was used to amplify regions corresponding to 680 bp of +29 and 370 bp of +32. These fragments were cloned upstream of the luciferase reporter
gene
in pGL2 basic (Promega) and
assayed
in transfection
assays
. Primer sequences are available...
Abstract
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