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  1. ...their true loci from nontranscribed pseudogenes. GeneWise (Birney and Durbin 1997 , 2000 ; Birney et al. 2004b ) is certainly the most important protein-to- alignment program, since it forms a central part of the highly influential Ensembl gene annotation pipeline. The accuracy of GeneWise is determined...
  2. ...) discussed the limitations associated with this assembly. A gene set annotated by VectorBase contains both manually annotated genes and predicted gene models from GeneWise (Birney et al. 2004), ClusterMerge (Eyras et al. 2004), and SNAP (Li et al. 2007) algorithms. The VectorBase bioinformatic resource...
  3. ....A. ( 2002 ) Planetary biology—Paleontological, geological, and molecular histories of life . Science 296 : 864 – 868 . ↵ Birney, E. , Clamp, M. , Durbin, R. ( 2004 ) GeneWise and Genomewise . Genome Res. 14 : 988 – 995 . ↵ Blank, L.M. , Lehmbeck, F. , Sauer, U. ( 2005 ) Metabolic-flux and network analysis...
  4. ...to identify complete genes ( Guigo et al. 2000 ). A new version of the program, called GenomeWise, uses cDNA and ESTs to define spliced gene structure. GenomeWise and GeneWise are used extensively in the Ensembl annotation pipeline ( Birney et al. 2004 ). Like most alignment methods, these programs...
  5. ...be pseudogenized, equivalent to gene loss. Using GeneWise ( Birney et al. 2004 ), we examined whether the open reading frame (ORF) was still intact for each downstream gene in the broken operons identified above, and found only two cases where the ORFs were disrupted. They are F46B6.6 in C. remanei and F37C12...
  6. ...date are at http:// www..org/cgi/doi/10.1101/gr.088997.108. 1912 Genome Research www..org 19:1912–1923; ISSN 1088-9051/09; www..org Results Data processing pipeline workflow The Gramene gene build is based on the Ensembl pipeline, but with several important modifications. First, while Ensembl uses GeneWise...
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