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  1. .... E. Bertling, W.M., Beier, F. and Reichenberger. E. 1993 . Determination of 5′ ends of specific mRNAs by DNA ligase-dependent amplification. PCR Methods Applic. 3 : 95 – 99 . 13.. Frohman, M.A. Frohman, M.A. 1993 . Rapid amplification of cDNA for generation of full-length cDNA ends: Thermal RACE...
  2. ...the size distribution of the cDNA library, removing the post-tagmentation PCR step necessary in nanoCAGE when ≥50 ng of total RNA are available, and thus minimizing the PCR cycles required for library amplification. In addition to the STRIPE-seq methodology, we provide an end-to-end computational workflow...
  3. ..., Morozov P, Ludwig J, et al. 2011. RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries. RNA 17. doi: 10.1261/rna.2799511. Langmead B, Trapnell C, Pop M, Salzberg SL. 2009. Ultrafast and memoryefficient alignment of short DNA sequences to the human . Genome Biol 10...
  4. ...protocol (Picelli et al. 2014). Reactions were incubated for 30 min at 37°C before isolation and purification of tagmented DNA using QIAquick MinElute columns (Qiagen). ATAC-seq libraries were prepared by PCR amplification using single index (i7) Illumina barcodes previously described (Buenrostro et al...
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