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  1. ...expression and other nuclear processes can be altered in disease. The constellation of transcription factors and chromatin regulatory complexes in cells results in characteristic patterns of chromatin accessibility. The study of these patterns in tissues has been limited because existing chromatin...
  2. ...with the following changes (Barski et al. 2007): Frozen left ventricular heart and liver tissuewas crunched using pestle and mortar; 125 mg tissue was homogenized in a Potter-Elvehjem tissue grinder, nuclei were isolated by centrifugation through a dense sucrose cushion and digested with micrococcal nuclease (Sigma...
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