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  1. .... and Gavilondo-Cowley. J.V. 1991 . Primer design for the cloning of immunoglobulin heavy chain leader-variable regions from mouse hybridoma cells using PCR. BioTechniques 11 : 152 – 156 . 37.. Brodeur, P.H., , Osman, G.E. , Mackle, J.J. , Lalor. T.M. Brodeur, P.H., Osman, G.E. Mackle, J.J. and Lalor. T.M. 1988...
  2. ...kilobaseBINDER M-280 Streptavidin beads were used instead of Dynabeads M270 Streptavidin beads. Postcapture PCR details are provided in Supplemental Methods S4. After postcapture PCR, only 300 ng of the cDNA sequencing library was prepared following the Ligation Sequencing Kit (Oxford Nanopore Technologies...
  3. ...A A Wellman Gumerlock P H P H, Gumerlock SJ DeNardo G L DeNardo Meyers, F.J F J Meyers F.J. Meyers A A, Wellman G.L. DeNardo genome;3/1/46 1088-9051 Rapid PCR construction of a gene containing Lym-1 antibody variable regions. Rapid PCR construction of a gene containing Lym-1 antibody variable regions. X B Shi...
  4. ...primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes. Eur. J. Immunol. 21 : 985 – 991 . 25. Sassano, M., Repetto, M. Cassani, G. and Corti. A. 1994 . PCR amplification of antibody variable regions using primers...
  5. ...temperatures. (4) During our study of rearranged murine immunoglobulin genes, we wished to analyze heavy- and light-chain variable regions from a number of hybridoma cell lines by PCR amplification and di- rect sequencing. We found that in some cases sequencing these PCR products di- rectly gave terminations...
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