Searching journal content for articles similar to Bautista et al. 4 (2): 76.

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  1. GENOME METHODS: A Streamlined Mutation Detection System: Multicolor Post-PCR Fluorescence Labeling and Single-Strand Conformational Polymorphism Analysis by Capillary Electrophoresis
    • Masakazu Inazuka,
    • Hans-Michael Wenz,
    • Munechika Sakabe,
    • Tomoko Tahira,
    • and Kenshi Hayashi
    Genome Res. November 1, 1997 7: 1094-1103; doi:10.1101/gr.7.11.1094
    ...in which fluorescently labeled PCR products are electrophoresed and detected by automated DNA sequencer ( Makino et al. 1992 ). This method is nonradioactive, is highly sensitive, and has the advantage of direct data storage and processing by the computer of the automated DNA sequencer. In this system...
  2. ARTICLES: A nonisotopic single-strand conformation polymorphism protocol using a direct blotting electrophoresis, a chemiluminescent detection system, and a multiplex approach.
    • H Knoblauch,
    • N Weiss,
    • I Eggersdorfer,
    • C Keller,
    • and H Schuster
    Genome Res. August 1994 4: 52-55
    .... Horn, G.T. Mullis, K.B. and Erlich. H.A. 1988 . Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239 : 487 – 491 . 24. Soto, D. and Sukumar. S. 1992 . Improved detection of mutations in the p53 gene in human tumors as single-stranded conformation polymorphs...
  3. REVIEW: PCR-based approaches for detection of mutated ras genes.
    • A D van Mansfeld and
    • J L Bos
    Genome Res. May 1992 1: 211-216; doi:10.1101/gr.1.4.211
    ...for mutations that can be located at different posi- tions in a DNA segment, such as the mutations occurring in the p53 gene. For such analyses, direct sequencing is also very suitable and the method most used. ACKNOWLEDGMENTS We thank D.H.J. van Weering for valu- able contributions and for Fig. 1 and Drs. P...
  4. REVIEW: PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA.
    • K Hayashi
    Genome Res. August 1991 1: 34-38; doi:10.1101/gr.1.1.34
    ...press). 17. Mashiyama, S., , Murakami, Y. , Yoshimoto, T. , Sekiya, T. , Hayashi. K. Mashiyama, S., Murakami, Y. Yoshimoto, T. Sekiya, T. and Hayashi. K. 1991 . Detection of p53 gene mutations in human brain tumors by single-strand conformation polymorphism analysis of polymerase chain reaction products...
  5. ARTICLES: F-SSCP: fluorescence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis.
    • R Makino,
    • H Yazyu,
    • Y Kishimoto,
    • T Sekiya,
    • and K Hayashi
    Genome Res. August 1992 2: 10-13; doi:10.1101/gr.2.1.10
    .... Screening of multiple samples for detection of sequence changes. Technique 2 : 304 – 306 . 9.. Mashiyama, S., , Murakami, Y. , Yoshimoto, T. , Sekiya, T. , Hayashi. K. Mashiyama, S., Murakami, Y. Yoshimoto, T. Sekiya, T. and Hayashi. K. 1991 . Detection of p53 gene mutations in human brain tumors by single...
  6. LETTER: Complex SNP-Based Haplotypes in Three Human Helicases: Implications for Cancer Association Studies
    • Dimitra Trikka,
    • Zhe Fang,
    • Alex Renwick,
    • Sally H. Jones,
    • Ranajit Chakraborty,
    • Marek Kimmel,
    • and David L. Nelson
    Genome Res. April 1, 2002 12: 627-639; doi:10.1101/gr.176702
    ...begun a candidate-gene approach and concentrated our efforts on detecting variation in genes that play a role in DNA maintenance and chromosomal integrity. We have utilized primarily noncoding sequence variants. The rationale for this choice was to avoid bias that might be introduced by studying sites...
  7. ARTICLES: Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP.
    • Q Liu and
    • S S Sommer
    Genome Res. October 1994 4: 97-108
    ...in a blinded manner and the results were compared with the "gold standard" of direct genomic se- quencing. All 36 mutations detected by genomic sequencing were also detected by ddF. A second blinded comparison of ddF and direct genomic sequencing de- tected 26 of 26 mutations in the p53 gene, a gene...
  8. ARTICLE: One-tube post-PCR fluorescent labeling of DNA fragments.
    • M Inazuka,
    • T Tahira,
    • and K Hayashi
    Genome Res. June 1996 6: 551-557; doi:10.1101/gr.6.6.551
    ...Electrophoresis The efficacy of the present labeling method in detect- ing mutat ion by SSCP analysis was also tested us- ing capillary electrophore- sis. Exon 8 of the p53 gene was ampl i f ied f rom ge- nomic DNA of THP-1, Na- malwa and Daudi cells, and used for labeling with R6G-dUTP after fourfold d i lu t...
  9. REVIEW: Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends
    • Deirdre Meldrum
    Genome Res. September 1, 2000 10: 1288-1303; doi:10.1101/gr.157400
    ...production ( Fodor et al. 1991 , 1997 ; Pease et al. 1994 ; Lipshutz et al. 1999 ). Some of the Affymetrix chips are commercially available and have been used, for example, to sequence the HIV PR and RT genes ( Jandreski 1995 ) and for TP53 mutation detection using the p53 GeneChip ( Wen et al. 2000...
  10. REVIEW: Multiplex PCR: advantages, development, and applications.
    • M C Edwards and
    • R A Gibbs
    Genome Res. February 1994 3: S65-S75
    .... Multiple polymerase chain reaction amplification and direct sequencing of homologous sequences: Point mutation of the ras gene. Anal. Biochem. 6 : 552 – 526 . 44. Clemens, P.R., Fenwick, R.G. Chamberlain, J.S. Gibbs, R.A. de Andrade, M. Chakraborty, R. and Caskey. C.T. 1991 . Carrier detection and prenatal...
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