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ARTICLE: Characterization of human AFLP systems apolipoprotein B, phenylalanine hydroxylase, and D1S80.
- D Latorra,
- C M Stern,
- and M S Schanfield
Genome Res. June 1994 3: 351-358
...Methods and Applications 353 action and thermal cycling parameters for APOB, PAH, and DIS80. Several con- centrations were found to be uniform for each system: [3-globin primer (0.05 p.M); dNTPs (200 I~M each), and agarose gel (2.0%). Key reaction variables were found to be template DNA amount...
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LETTER: A Comprehensive Analysis of Allelic Methylation Status of CpG Islands on Human Chromosome 21q
- Yoichi Yamada,
- Hidemi Watanabe,
- Fumihito Miura,
- Hidenobu Soejima,
- Michiko Uchiyama,
- Tsuyoshi Iwasaka,
- Tsunehiro Mukai,
- Yoshiyuki Sakaki,
- and Takashi Ito
Genome Res. February 2004 14: 247-266; doi:10.1101/gr.1351604
...sulfoxide (Sigma), or PCR enhancer (Invitrogen) was added to the reaction mixture to improve amplification ( Baskaran et al. 1996 ). The thermal cycling parameter was optimized for each amplicon. See Supplemental Table S1 for detailed conditions for each PCR including primer sequences. The amplified...
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Methods: DNA Mapping Using Microfluidic Stretching and Single-Molecule Detection of Fluorescent Site-Specific Tags
- Eugene Y. Chan,
- Nuno M. Goncalves,
- Rebecca A. Haeusler,
- Amie J. Hatch,
- Jonathan W. Larson,
- Anthony M. Maletta,
- Gregory R. Yantz,
- Eugene D. Carstea,
- Martin Fuchs,
- Gordon G. Wong,
- Steven R. Gullans,
- and Rudolf Gilmanshin
Genome Res. June 2004 14: 1137-1146; doi:10.1101/gr.1635204
..., a funnel with a 10:1 taper reduction ratio, a taper shape providing W(x)∼1/x2 profile (W is the channel width, and x is the coordinate along the flow direction), and a 5 μm-wide interrogation channel. The interrogation channel had uniform cross-section to ensure constant solution velocity, which was 10...
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ARTICLE: High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity.
- F C Lawyer,
- S Stoffel,
- R K Saiki,
- S Y Chang,
- P A Landre,
- R D Abramson,
- and D H Gelfand
Genome Res. May 1993 2: 275-287; doi:10.1101/gr.2.4.275
...efficiently, Taq Pol I yields long stretches of readable DNA sequence that are uniform in intensity and free of background. ~4~ A 94-kD Taq Pol I has been purified from Taq, but growing the organism is more difficult than growing Escherichia coll. Although the activity yield is high (40--60%), the expression...
ARTICLE: The use of the PCR to quantitate gene expression.
- W C Gause and
- J Adamovicz
Genome Res. June 1994 3: S123-S135
...C Gause and J Adamovicz Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814. Footnotes Copyright © Cold Spring Harbor Laboratory Press References 1.. Murphy, L.D., , Herzog, C.E. , Rudick, J.B. , Fojo, A.T. , Bates. S.E. Murphy, L.D., Herzog, C...
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LETTER: Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution of Yeast Strains
- José E. Pérez-Ortı́n,
- Amparo Querol,
- Sergi Puig,
- and Eladio Barrio
Genome Res. October 1, 2002 12: 1533-1539; doi:10.1101/gr.436602
...uniform ( Pretorius 2000 ). Their exacerbated capacity to reorganize its by chromosome rearrangements such as Ty-promoted chromosomal translocations ( Longo and Vézinhet 1993 ; Rachidi et al. 1999 ), mitotic crossing-over ( Aguilera et al. 2000 ), and gene conversion ( Puig et al. 2000 ) promotes a faster...
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LETTER: Genome Size Evolution in Pufferfish: A Comparative Analysis of Diodontid and Tetraodontid Pufferfish Genomes
- Daniel E. Neafsey and
- Stephen R. Palumbi
Genome Res. May 1, 2003 13: 821-830; doi:10.1101/gr.841703
...per base pair in the smooth and spiny alignments was 0.016 and 0.021, respectively. Assuming a time-uniform nucleotide substitution rate on the order of 10−9 per base pair per generation and a 1.5-yr generation time indicates that on average the sequences in these alignments have been neutrally...

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