Searching journal content for articles similar to Auer et al. 24 (1): 142.

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  1. Method: Efficient and flexible tagging of endogenous genes by homology-independent intron targeting
    • Yevgeniy V. Serebrenik,
    • Stephanie E. Sansbury,
    • Saranya Santhosh Kumar,
    • Jorge Henao-Mejia,
    • and Ophir Shalem
    Genome Res. August 2019 29: 1322-1328; Published in Advance June 25, 2019, doi:10.1101/gr.246413.118
    ...Protoc 2: 1515–1527. doi:10.1038/nprot.2007.197 ↵Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, et al. 2016. In vivo editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature 540: 144–149. doi:10.1038/nature20565 ↵Trinh le...
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  2. Method: Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases
    • Séverine Remy,
    • Laurent Tesson,
    • Séverine Menoret,
    • Claire Usal,
    • Anne De Cian,
    • Virginie Thepenier,
    • Reynald Thinard,
    • Daniel Baron,
    • Marine Charpentier,
    • Jean-Baptiste Renaud,
    • Roland Buelow,
    • Gregory J. Cost,
    • Carine Giovannangeli,
    • Alexandre Fraichard,
    • Jean-Paul Concordet,
    • and Ignacio Anegon
    Genome Res. August 2014 24: 1371-1383; Published in Advance July 2, 2014, doi:10.1101/gr.171538.113
    ...now join ZFNs as having effected targeted transgene integration in vivo. CRISPR/Cas9 are even easier to Table 1. Microinjection statistics for the Hprt1.1, Hprt 1.2, and Rosa26 loci Target locus Dose mRNA/DNA (ng/µl) Temperature No. injected eggs (% viable eggs) No. E15 (e) or pups (p) (%)a No. of GFP...
  3. Method: A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor
    • Hengxing Lu,
    • Jun Liu,
    • Tao Feng,
    • Zihang Guo,
    • Yunjun Yin,
    • Fei Gao,
    • Gengsheng Cao,
    • Xuguang Du,
    • and Sen Wu
    Genome Res. May 2021 31: 900-909; Published in Advance April 1, 2021, doi:10.1101/gr.271312.120
    ...CRISPR/Cas9-induced homology-dependent and independent DNA repair. Nucleic Acids Res 44: e85. doi:10.1093/nar/gkw064 ↵Hsu PD, Lander ES, Zhang F. 2014. Development and applications of CRISPR-Cas9 for engineering. Cell 157: 1262–1278. doi:10.1016/j.cell.2014.05.010 ↵Ishida Y, Leder P. 1999. RET: a poly A...
  4. Method: 2C-Cas9: a versatile tool for clonal analysis of gene function
    • Vincenzo Di Donato,
    • Flavia De Santis,
    • Thomas O. Auer,
    • Noé Testa,
    • Héctor Sánchez-Iranzo,
    • Nadia Mercader,
    • Jean-Paul Concordet,
    • and Filippo Del Bene
    Genome Res. May 2016 26: 681-692; Published in Advance March 8, 2016, doi:10.1101/gr.196170.115
    ...contributed equally to this work. ↵5 Present address: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland AbstractCRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date...
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