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  1. ...distribution in PH-1 and Δash1 around H3K27me3-marked genes (±2.0 kb). (D) Venn diagrams of overlap between H3K27me3-marked genes and Ash1-dependent H3K36me3-marked genes. (E) GO enrichment analysis of overlapping genes from D (n = 1092). (F,G) Heat maps of loci with increased (F) or decreased (G) H3K27me3...
  2. ..., of duplicated gene expression according to the histone modifications present, we examined -wide patterns of histone modifications in our animal models. Histone modification data can be obtained with chromatin immunoprecipitation combined with DNA microarray analysis (ChIP-chip) (e.g., Bernstein et al. 2005...
  3. ...Chromatin immunoprecipitation profiling of H3K27ac and H3K27me3 marks further revealed 168 distinct patterns of histone modification following immune stimulation. Stimulated fish 169 showed an average of 72,490 (±21,211) H3K27ac and 68,873 (±16,608) H3K27me3 peaks, 170 compared to 78,816 (±31,106) and 48...
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  4. ...known factors involved in the epigenetic control of IES excision (Fig. 4E,F; Supplemental Fig. S11). We found an underrepresentation of the very early excised cluster among the subset of IESs whose excision depends on the deposition of H3K9me3 and H3K27me3 marks (i.e., IESs retained in an EZL1 KD...
  5. ...and genes proximal to these regions. On the other hand, the repressive polycomb-mediated H3K27me3 mark, also profiled in adults, was enriched at negatively correlated peaks, consistent with H3K27me3 regions marking silencers (Fig. 2D; Cai et al. 2021). Linking this corroborating information to developmental...
  6. ...by the observation that genes induced by Aire expression are generally characterized by a repressive chromatin state in somatic tissues, we found these genes to be strongly associated with H3K27me3 marks in mTEC. Our findings are consistent with AIRE targeting and inducing the promiscuous expression of genes...
  7. ...ac, and bore the combination of active H3K4me3 and repressive H3K27me3 marks typical of bivalent promoters, and no H3K36me3 over their gene body. Interdependence between TRIM28 ERE recruitment and DNA methylation In murine ES cells, TRIM28-induced repression leads to DNA methylation, which is thought...
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