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Research: Revisiting chromatin packaging in mouse sperm
- Qiangzong Yin,
- Chih-Hsiang Yang,
- Olga S. Strelkova,
- Jingyi Wu,
- Yu Sun,
- Sneha Gopalan,
- Liyan Yang,
- Job Dekker,
- Thomas G. Fazzio,
- Xin Zhiguo Li,
- Johan Gibcus,
- and Oliver J. Rando
Genome Res. December 2023 33: 2079-2093; Published in Advance December 21, 2023, doi:10.1101/gr.277845.123
...IP-seq (Johnson et al. 2007) on formaldehyde-fixed sperm chromatin sheared via sonication (Methods) (Fig. 5; Supplemental Fig. S6). We focused on a sequence-specific DNA-binding protein, CTCF, and the histone modifications H3K4me3 and H3K27me3 (K4/27), as these epitopes were expected to show distinctive...
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Research: Genes for embryo development are packaged in blocks of multivalent chromatin in zebrafish sperm
- Shan-Fu Wu,
- Haiying Zhang,
- and Bradley R. Cairns
Genome Res. April 2011 21: 578-589; Published in Advance March 7, 2011, doi:10.1101/gr.113167.110
...chromatin (Boyer et al. 2005, 2006a,b; Orkin 2005;Welstead et al. 2008). Another outstanding question is how these regions are bounded, as the boundaries for this chromatin are often quite sharp, raising the possibility of a boundary factor such as CTCF. Further work in this area will involve an examination...
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