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Method: Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants
- Iraad F. Bronner,
- Thomas D. Otto,
- Min Zhang,
- Kenneth Udenze,
- Chengqi Wang,
- Michael A. Quail,
- Rays H.Y. Jiang,
- John H. Adams,
- and Julian C. Rayner
Genome Res. July 2016 26: 980-989; Published in Advance May 10, 2016, doi:10.1101/gr.200279.115
...Resources). References Alsford S, Turner DJ, Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C, Horn D. 2011. High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Genome Res 21: 915–924. Ariey F, Witkowski B, Amaratunga C, Beghain J...
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LETTER: Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16-kb GC-rich transcriptional “strand-switch” domain as a major feature
- Samson O. Obado,
- Martin C. Taylor,
- Shane R. Wilkinson,
- Elizabeth V. Bromley,
- and John M. Kelly
Genome Res. January 2005 15: 36-43; doi:10.1101/gr.2895105
...with other trypanosomatids, including Leishmania and the African trypanosome T. brucei , this parasite exhibits several unusual genetic traits. Protein-coding genes have no recognizable RNA polymerase II (pol II)-specific promoters, transcription is polycistronic, and all mRNAs are modified post...

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