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Resource: The completion of the Mammalian Gene Collection (MGC)
- The MGC Project Team,
- Gary Temple,
- Daniela S. Gerhard,
- Rebekah Rasooly,
- Elise A. Feingold,
- Peter J. Good,
- Cristen Robinson,
- Allison Mandich,
- Jeffrey G. Derge,
- Jeanne Lewis,
- Debonny Shoaf,
- Francis S. Collins,
- Wonhee Jang,
- Lukas Wagner,
- Carolyn M. Shenmen,
- Leonie Misquitta,
- Carl F. Schaefer,
- Kenneth H. Buetow,
- Tom I. Bonner,
- Linda Yankie,
- Ming Ward,
- Lon Phan,
- Alex Astashyn,
- Garth Brown,
- Catherine Farrell,
- Jennifer Hart,
- Melissa Landrum,
- Bonnie L. Maidak,
- Michael Murphy,
- Terence Murphy,
- Bhanu Rajput,
- Lillian Riddick,
- David Webb,
- Janet Weber,
- Wendy Wu,
- Kim D. Pruitt,
- Donna Maglott,
- Adam Siepel,
- Brona Brejova,
- Mark Diekhans,
- Rachel Harte,
- Robert Baertsch,
- Jim Kent,
- David Haussler,
- Michael Brent,
- Laura Langton,
- Charles L.G. Comstock,
- Michael Stevens,
- Chaochun Wei,
- Marijke J. van Baren,
- Kourosh Salehi-Ashtiani,
- Ryan R. Murray,
- Lila Ghamsari,
- Elizabeth Mello,
- Chenwei Lin,
- Christa Pennacchio,
- Kirsten Schreiber,
- Nicole Shapiro,
- Amber Marsh,
- Elizabeth Pardes,
- Troy Moore,
- Anita Lebeau,
- Mike Muratet,
- Blake Simmons,
- David Kloske,
- Stephanie Sieja,
- James Hudson,
- Praveen Sethupathy,
- Michael Brownstein,
- Narayan Bhat,
- Joseph Lazar,
- Howard Jacob,
- Chris E. Gruber,
- Mark R. Smith,
- John McPherson,
- Angela M. Garcia,
- Preethi H. Gunaratne,
- Jiaqian Wu,
- Donna Muzny,
- Richard A. Gibbs,
- Alice C. Young,
- Gerard G. Bouffard,
- Robert W. Blakesley,
- Jim Mullikin,
- Eric D. Green,
- Mark C. Dickson,
- Alex C. Rodriguez,
- Jane Grimwood,
- Jeremy Schmutz,
- Richard M. Myers,
- Martin Hirst,
- Thomas Zeng,
- Kane Tse,
- Michelle Moksa,
- Merinda Deng,
- Kevin Ma,
- Diana Mah,
- Johnson Pang,
- Greg Taylor,
- Eric Chuah,
- Athena Deng,
- Keith Fichter,
- Anne Go,
- Stephanie Lee,
- Jing Wang,
- Malachi Griffith,
- Ryan Morin,
- Richard A. Moore,
- Michael Mayo,
- Sarah Munro,
- Susan Wagner,
- Steven J.M. Jones,
- Robert A. Holt,
- Marco A. Marra,
- Sun Lu,
- Shuwei Yang,
- James Hartigan,
- Marcus Graf,
- Ralf Wagner,
- Stanley Letovksy,
- Jacqueline C. Pulido,
- Keith Robison,
- Dominic Esposito,
- James Hartley,
- Vanessa E. Wall,
- Ralph F. Hopkins,
- Osamu Ohara,
- and Stefan Wiemann
Genome Res. December 2009 19: 2324-2333; Published in Advance September 18, 2009, doi:10.1101/gr.095976.109
...material and at ftp://ftp.ncbi.nih.gov/repository/MGC/MGC_project/.] cDNA clones containing the entire protein-coding sequence of mRNA transcripts (full-CDS clones), together with corresponding high-quality sequences, are essential resources for annotating protein-coding genes on s and for expressing...
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Method: CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for more than 5500 Drosophila genes conserved in humans
- Ping Wei,
- Wen Xue,
- Yun Zhao,
- Guang Ning,
- and Jiwu Wang
Genome Res. January 2020 30: 95-106; Published in Advance November 13, 2019, doi:10.1101/gr.250811.119
..., mouse, and human cDNA and ORF clones used in this study are from the Drosophila Genome Resource Center (https://dgrc.bio.indiana.edu) Gold Collection, the mouse Mammalian Gene Collection (MGC, https://genecollections.nci.nih.gov/MGC/), and the human CCSB-Broad Lentiviral Expression Library (Yang et al...
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RESOURCE: Sequencing and analysis of 10,967 full-length cDNA clones from Xenopus laevis and Xenopus tropicalis reveals post-tetraploidization transcriptome remodeling
- Ryan D. Morin,
- Elbert Chang,
- Anca Petrescu,
- Nancy Liao,
- Malachi Griffith,
- Robert Kirkpatrick,
- Yaron S. Butterfield,
- Alice C. Young,
- Jeffrey Stott,
- Sarah Barber,
- Ryan Babakaiff,
- Mark C. Dickson,
- Corey Matsuo,
- David Wong,
- George S. Yang,
- Duane E. Smailus,
- Keith D. Wetherby,
- Peggy N. Kwong,
- Jane Grimwood,
- Charles P. Brinkley III,
- Mabel Brown-John,
- Natalie D. Reddix-Dugue,
- Michael Mayo,
- Jeremy Schmutz,
- Jaclyn Beland,
- Morgan Park,
- Susan Gibson,
- Teika Olson,
- Gerard G. Bouffard,
- Miranda Tsai,
- Ruth Featherstone,
- Steve Chand,
- Asim S. Siddiqui,
- Wonhee Jang,
- Ed Lee,
- Steven L. Klein,
- Robert W. Blakesley,
- Barry R. Zeeberg,
- Sudarshan Narasimhan,
- John N. Weinstein,
- Christa Prange Pennacchio,
- Richard M. Myers,
- Eric D. Green,
- Lukas Wagner,
- Daniela S. Gerhard,
- Marco A. Marra,
- Steven J.M. Jones,
- and Robert A. Holt
Genome Res. June 2006 16: 796-803; Published in Advance May 3, 2006, doi:10.1101/gr.4871006
....A. , Shenmen C.M. , Grouse L.H. , Schuler G. , Klein S.L. , Old S. , Rasooly R. , Good P. , et al. ( 2004 ) The status, quality, and expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 14 : 2121 – 2127 . ↵ Gilchrist M.J. , Zorn A.M. , Voigt J. , Smith J...
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Methods: Systematic Recovery and Analysis of Full-ORF Human cDNA Clones
- Ágnes Baross,
- Yaron S.N. Butterfield,
- Shaun M. Coughlin,
- Thomas Zeng,
- Malachi Griffith,
- Obi L. Griffith,
- Anca S. Petrescu,
- Duane E. Smailus,
- Jaswinder Khattra,
- Helen L. McDonald,
- Sheldon J. McKay,
- Michelle Moksa,
- Robert A. Holt,
- and Marco A. Marra
Genome Res. October 15, 2004 14: 2083-2092; doi:10.1101/gr.2473704
...the structure of genes including identification of splice variants, and understanding the proteome. Furthermore, access to the cDNA clones would facilitate functional studies of genes and corresponding proteins. The Mammalian Gene Collection (MGC; http://mgc.nci.nih.gov ) project aims to generate a public...
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Methods: Gene prediction and verification in a compact genome with numerous small introns
- Aaron E. Tenney,
- Randall H. Brown,
- Charles Vaske,
- Jennifer K. Lodge,
- Tamara L. Doering,
- and Michael R. Brent
Genome Res. November 2004 14: 2330-2335; Published in Advance October 12, 2004, doi:10.1101/gr.2816704
.... Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. 99 : 16899 -16903. ↵ The MGC Project Team. 2004 . The status, quality and expansion of the NIH full-length cDNA project: The mammalian gene collection (MGC). Genome Res. 14 : 2121 -2127...
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LETTER: Multiple Variable First Exons: A Mechanism for Cell- and Tissue-Specific Gene Regulation
- Theresa Zhang,
- Peter Haws,
- and Qiang Wu
Genome Res. January 2004 14: 79-89; Published in Advance December 12, 2003, doi:10.1101/gr.1225204
...Genomic Organizations Exist in Mammalian Genomes? Large-scale genomic sequencing has outpaced our ability to annotate sequences. To date, sequencing cDNA clones is still the most reliable method to identify genes. Although EST sequencing has provided valuable information about annotation, it usually does...
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LETTER: 1274 Full-Open Reading Frames of Transcripts Expressed in the Developing Mouse Nervous System
- Maria F. Bonaldo,
- Thomas B. Bair,
- Todd E. Scheetz,
- Einat Snir,
- Ike Akabogu,
- Jennifer L. Bair,
- Brian Berger,
- Keith Crouch,
- Aja Davis,
- Mari E. Eyestone,
- Catherine Keppel,
- Tamara A. Kucaba,
- Mark Lebeck,
- Jenny L. Lin,
- Anna I.R. de Melo,
- Joshua Rehmann,
- Rebecca S. Reiter,
- Kelly Schaefer,
- Christina Smith,
- Dylan Tack,
- Kurtis Trout,
- Val C. Sheffield,
- Jim J-C. Lin,
- Thomas L. Casavant,
- and Marcelo B. Soares
Genome Res. October 15, 2004 14: 2053-2063; doi:10.1101/gr.2601304
...Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length...
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LETTER: Comparative Analysis of Gene Expression for Convergent Evolution of Camera Eye Between Octopus and Human
- Atsushi Ogura,
- Kazuho Ikeo,
- and Takashi Gojobori
Genome Res. August 2004 14: 1555-1561; doi:10.1101/gr.2268104
.... To compare the expressed genes between human and octopus eyes, using the BodyMap data and the human sequence data, we obtained ∼1800 genes that were expressed in human eyes (retina, cornea, etc.). MGC, the Mammalian Gene Collection ( Strausberg et al. 1999 ) maintained by NIH, also has a similar data set...
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Methods: Targeted discovery of novel human exons by comparative genomics
- Adam Siepel,
- Mark Diekhans,
- Broňa Brejová,
- Laura Langton,
- Michael Stevens,
- Charles L.G. Comstock,
- Colleen Davis,
- Brent Ewing,
- Shelly Oommen,
- Christopher Lau,
- Hung-Chun Yu,
- Jianfeng Li,
- Bruce A. Roe,
- Phil Green,
- Daniela S. Gerhard,
- Gary Temple,
- David Haussler,
- and Michael R. Brent
Genome Res. December 2007 17: 1763-1773; Published in Advance November 7, 2007, doi:10.1101/gr.7128207
...that are as complete and accurate as possible, by some reasonable working definition of the gene. Also needed are essential reagents related to genes, such as cDNA clones. The Mammalian Gene Collection (MGC) project was created to provide the community with a representative high-quality full-length cDNA clone...
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LETTER: Analysis of Human mRNAs With the Reference Genome Sequence Reveals Potential Errors, Polymorphisms, and RNA Editing
- Terrence S. Furey,
- Mark Diekhans,
- Yontao Lu,
- Tina A. Graves,
- Lachlan Oddy,
- Jennifer Randall-Maher,
- LaDeana W. Hillier,
- Richard K. Wilson,
- and David Haussler
Genome Res. October 15, 2004 14: 2034-2040; doi:10.1101/gr.2467904
...human and mouse cDNA sequences. Proc. Natl. Acad. Sci. 99 : 16899 -16903. ↵ MGC Project Team. 2004 . The status, quality, and expansion of the NIH full-length cDNA project: The mammalian gene collection (MGC). Genome Res. (this issue). ↵ Miyazaki, S., Sugawara, H., Ikeo, K., Gojobori, T., and Tateno, Y...

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