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  1. ...then scraped and diluted in water at 1 OD600. The yeast suspension was serially diluted by 10-fold, and 3 L was spotted on different media (YP medium supplemented with glucose, galactose, ethanol, or glycerol) or UV-irradiated at 5 or 20 J/m2 (UV Stratalinker 1800) and incubated for 3 d or 5 d ...
  2. OPEN ACCESS ARTICLE
  3. ...in its 3-untranslated region (pJM101/ L1.3mneoI ) ( Dombroski et al. 1993 ; Sassaman et al. 1997 ). The retrotransposition cassette consists of a backward copy of the neomycin phosphotransferase gene containing its own promoter and polyadenylation signal ( Jensen and Heidmann 1991 ; Freeman et al. 1994 ...
  4. ..., N., 837 Ajay, S.S., 1407 Akagi, K., 870 Akalin, A., 486 Akdis, C.A., 1050 Aken, B.L., 1698, 1760 Akey, J.M., 1689 Akimitsu, N., 947 Alani, E., 714 Alba, M.M., 478 Alber, F., 1295 Alekseyenko, A.A., 2188 Alexa, K., 1541 Alexander, R., 1658 Alexis, M.S., 2356 Alisch, R.S., 623, 1395 Alkan, C., 778 ...
  5. ...LaJVM cells for insertion #1 and insertion #7. JM101 is a retrotransposition-competent human L1 (L1.3); JM105 is a negative control, consisting of L1.3 with a reverse transcriptase active site mutation. TGF21 is an active GF subfamily mouse L1; L1spa is an active TF subfamily mouse L1. Colony formation ...
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