A chromosome-specific microdissected library increases marker density on bovine chromosome 1.

Table 2. Type II loci (ms) generated from clones obtained from the BTA1 library.

Locus Foreward Primer Sequence (5' ->3') Reverse Primer Sequence (5' ->3') Anneal. Temp. Alleles Max. Alleles Min. Alleles No. Informative Meioses Genbank Acc. #
BMS4000 TCCCTCCTTGATGGATTCAG TTGAAGTAGCCTGAGAAAAGGG 56 147 117 12 329 G18483
BMS4001 AGCTCTTTGCTTCCTTAAGCC GAGGTGCTCAGAGAAGGCC 58 124 104 7 197 G19031
BMS4002 ATGGTAATGAGGATGGAGGAG TGATATCCTTGATTTCTCTATCTCC 54 125 105 6 217 G19032
BMS4003 TTCACCGATTTTACCCTTTTTCC TGGCATCAACAAGCTGCC 58 92 80 6 119 G19029
BMS4004 TTCAATGGAAAACCAATAAAGG ATACAACCTCTCCACCTCAAAC 58 163 149 6 233 G19081
BMS4006 CATCCATGTTGTTGCAAATAGC ATATAGTGTGGGTGTGGGTGTG 60 128 110 10 269 G19071
BMS4007 AACAAAACTCACCTCCCCG AATAGTTTGGGCCATCATTCC 58 203 197 3 247 G19030
BMS4008 CGGCCCTAAGTGATATGTTG GAAGAGTGTGAGGGAAAGACTG 58 176 150 8 292 G19065
BMS4009 TGGCAAGCTACAGTCCACAG TATGTTTCTGCATTGGCAGG 62 165 151 8 237 G19066
BMS4010 AGTGGAACCAAATAAAGAACCC ATAGCAAAAGAATACTTCCCCC 58 148 138 6 272 G19067
BMS4011 TGAAGCTGACATTTCCACATG CTTCCAGGCAACTAAATCAACC 58 208 192 8 218 G18510
BMS4012 TCCCTCCCTCCTTATGCAC CGCAAGTAACCATGCCATC 58 121 105 8 247 G19068
BMS4013a TTATTCTACATTGCAACCCAGG GTCGGACATGACTGAGCAAC 56 96 78 9 318 G19069
BMS4014a GACACGACTGAGCGACTGAG AAAGGAGCCTAAAAACACATCG 58 87 73 8 255 G19070
BMS4015 GGGTACATTGTTTTGCAGTTTG CAGGCTATAGTCCATGAGGTTG 58 156 130 9 267 G19057
BMS4016 CCAATCTGACTGCATAACGG AGGGCTCTCTTCTCAGCTTG 58 148 138 3 157 G19058
BMS4017 TATTCCTGAAAGACCTCCTCAC TGTGCATTTTTCTCTTCATCTC 58 163 143 7 241 G19059
BMS4018 GTCTTGGGGTTGCAGAGATC CCCTCACAGAGCTAGAGATATGC 54 99 97 2 58 G19060
BMS4019a GGGTCACAAAGAGTCAGACATG CTTCTGGTGCCATAATCTTGG 60 199 197 2 63 G19061
BMS4020 GAGCAGTCTGATGGGCTACA TGCCTTCTTTTCTGTCTGCA 58 116 114 2 181 G19087
BMS4021a GGGTCGCAAAGAATCAGAC GCTAGTTGCTGAAATTGGATTC 56 170 112 14 324 G19053
BMS4023 CGCATAGGGGTGTGTGTG ATGCTATGTTGAAAAATGGTGC 58 109 107 2 51 G19062
BMS4024 CAGGTTTAAAAACAAAGCAGAAG CATTCCTGTCATATCTTATCTGCTC 58 215 181 9 287 G19082
BMS4025 CACATCACAAATCCCTGCAC TCTTTGGATCAAACCCGAAC 58 107 97 5 228 G19054
BMS4027b TGGCCCAAGGAAAAATTTAG AGGAGTTGGCATGATTAGCTC 58 167 165 3 34 G19063
BMS4028 TGGGGTTGAAAAAGAACTGG ATAAAGCAGATGTGGTGTGTGC 58 120 92 9 257 G19064
BMS4029 AGAACGGGGCTGACTGTG AAACGGTCCCTGCAACAG 58 179 159 3 63 G19052
BMS4030 TGTACCCAACACAGGAGCAC TGACAGAGGGACCCATATCC 58 180 162 8 253 G19083
BMS4031 TCTTGCTGAACAAAGGTTCC TCCCAGGTATTTGAAGTGTTTC 58 300 278 9 319 G19088
BMS4033 GCCTGGTGGGATATGGTCC GCTCAAATGCCCGGTTTTC 58 175 171 2 126 G19084
BMS4035 AACAACCGTGAAAGAGCAAG GAACCTGCATCATGAAAAGC 54 176 162 7 251 G19056
BMS4036c GCTCCCAATAAGAAGGTCCC GGATCGAGACTTAAGAACACGC 56 101 95 3 132 G19047
BMS4037 CCCCCATAATGCTACATATTGC TTAAGACATGAATCCTCAGGGC 58 178 162 8 260 G19048
BMS4038 GAAAGTGCCAAAAGAAATTGC CCCATCCGTAGGTATGAAATG 58 137 119 7 329 G19049
BMS4039 CTGCAGCAAAAACCCAGG GGCTGGGAAAACTGTCTGG 58 130 116 6 280 G19085
BMS4040d GTCCATAGGGTCACACAGAGTC CCAAATCTTACCATAGCAAAGG 56 110 84 9 232 G19076
BMS4041a TAACAGAGGAGCCTGGCG TTTGGATCATTTTGCAGTGTG 60 230 224 5 257 G19077
BMS4043 TTACAGAAAGAGTGTGTGTGCG GGCTACAGTTCACAGGTTGC 58 117 99 7 217 G19078
BMS4044 TGGATTCTTAACCACTGGGC TTTCCTTACATTTTGACTGTCCC 56 160 132 13 325 G19079
BMS4045 TTTTTATTTTGGCTATCCTTGG ATGCCTGTAGGTTAGGAAAGTG 58 134 104 7 205 G19080
BMS4047 GAGAGGGCACTGGTTTCTTG TGTTGGACACGGATAAAAAGC 58 250 248 2 33 G19072
BMS4048 AGGTCCCTCACAAAATGGC AGTCCCAGGTTGCTGAAATG 58 145 125 7 165 G19073
BMS4049 GATCAAGTTGTCAACACACACAC TCTCATTTCTCTCCCTGTGC 56 111 91 7 322 G19074
BMSB4049e GATCAAGTTGTCAACACACACAC TCTCATTTCTCTCCCTGTGC 56 85 75 4 179 G19074
BMS4050 TGCTGAAGAAAAAAAGGGAC GTTTCAGGGCATTTAGACTCTG 58 152 104 13 356 G19089
BMS4051 TGTTTAGACATCCATCACATGC AATCGGTTGCCTTGATAATATG 58 135 135 1 0 G19075
BMS4052 AGAATCTCTTCCATTCCCTGTG CAAAAGGCAAAACAGAAATGC 56 185 171 6 150 G19086

a One primer sequence is similar to A or C SINEs (>85%).
b Locus genotypes link marker to BTA29.
c Locus genotypes link marker to BTA23.
d Once primer sequence is similar to LINEs (>85%).
e Secondary amplification product from BMS4049 that links marker to BTA6.


This Article

  1. doi: 10.1101/gr.7.1.76 Genome Res. January 1997 vol. 7 no. 1 76-80
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