Abstract
Cancer genomes frequently carry APOBEC (apolipoprotein B mRNA editing catalytic polypeptide-like)-associated DNA mutations, suggesting APOBEC enzymes as innate mutagens during cancer initiation and evolution. However, the pure mutagenic impacts of the specific enzymes among this family remain unclear in human normal cell lineages. Here, we investigated the comparative mutagenic activities of APOBEC3A and APOBEC3B, through whole-genome sequencing of human normal gastric organoid lines carrying doxycycline-inducible APOBEC expression cassettes. Our findings demonstrated that transcriptional upregulation of APOBEC3A led to the acquisition of a massive number of genomic mutations in just a few cell cycles. By contrast, despite clear deaminase activity and DNA damage, APOBEC3B upregulation did not generate a significant increase in mutations in the gastric epithelium. APOBEC3B-associated mutagenesis remained minimal even in the context of TP53 inactivation. Further analysis of the mutational landscape following APOBEC3A upregulation revealed a detailed spectrum of APOBEC3A-associated mutations, including indels, primarily 1 bp deletions, clustered mutations, and evidence of selective pressures acting on cells carrying the mutations. Our observations provide a clear foundation for understanding the mutational impact of APOBEC enzymes in human cells.