A novel synthetic-genetic-array based yeast one-hybrid system for high discovery rate and short processing time
- Chung-Shu Yeh1,
- Zhifeng Wang2,
- Fang Miao3,
- Hongyan Ma2,
- Chung-Ting Kao3,
- Tzu-Shu Hsu3,
- Jhong-He Yu3,
- Er-Tsi Hung3,
- Chia-Chang Lin3,
- Chen-Yu Kuan3,
- Ni-Chiao Tsai3,
- Chenguang Zhou2,
- Guan-Zheng Qu2,
- Jing Jiang2,
- Guifeng Liu2,
- Jack P Wang4,
- Wei Li2,
- Vincent L Chiang2,
- Tien-Hsien Chang1 and
- Ying-Chung Lin5,6
Abstract
The eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cis-targets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the Synthetic Genetic Array analysis. The system has a transcription factor-DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF-DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms.
- Received November 2, 2018.
- Accepted June 6, 2019.
- Published by Cold Spring Harbor Laboratory Press
This manuscript is Open Access.
This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International license), as described at http://creativecommons.org/licenses/by-nc/4.0/.
