Crosstalk between alternative polyadenylation and miRNA in regulation of protein translational efficiency
Abstract
3'UTRs play important roles in the gene regulation network via their influence on mRNA stability, translational efficiency and subcellular localization. For a given gene, 3'UTRs of different lengths generated by alternative polyadenylation (APA) may result in functional differences in regulation. The mechanistic details of how length changes of 3'UTRs alter gene function remains unclear. By combining APA sequencing and polysome profiling, we observed that mRNA isoforms with shorter 3'UTRs were bound with more polysomes in six cell lines but not in NIH3T3 cells, suggesting that changing 3'UTRs to shorter isoforms may lead to a higher gene translational efficiency. By interfering with the expression of GW182 and analyzing AGO2-PAR-CLIP data, we revealed that the APA effect on translational efficiency was mainly regulated by miRNA and that this regulation was cell cycle dependent. The discrepancy between NIH3T3 and other cell lines was due to contact inhibition of NIH3T3. Thus, crosstalk between APA and miRNA may be needed for the regulation of protein translational efficiency.
- Received October 24, 2017.
- Accepted September 11, 2018.
- Published by Cold Spring Harbor Laboratory Press
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